{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:08:40Z","timestamp":1790838520811,"version":"4.1.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:p>It is well established that motility is an essential virulence trait of the human gastric pathogen<jats:italic>Helicobacter pylori<\/jats:italic>. Accordingly, chemotaxis contributes to the ability of<jats:italic>H. pylori<\/jats:italic>to colonize animal infection models. Chemotactic signal transduction in<jats:italic>H. pylori<\/jats:italic>differs from the enterobacterial paradigm in several respects. In addition to a separate CheY response regulator protein (CheY1),<jats:italic>H. pylori<\/jats:italic>contains a CheY-like receiver domain (CheY2) which is C-terminally fused to the histidine kinase CheA. Furthermore, the genome of<jats:italic>H. pylori<\/jats:italic>encodes three CheV proteins consisting of an N-terminal CheW-like domain and a C-terminal receiver domain, while there are no orthologues of the chemotaxis genes<jats:italic>cheB<\/jats:italic>,<jats:italic>cheR<\/jats:italic>and<jats:italic>cheZ<\/jats:italic>. To obtain insight into the mechanisms controlling the chemotactic response of<jats:italic>H. pylori<\/jats:italic>, we investigated the phosphotransfer reactions between the purified two-component signalling modules<jats:italic>in vitro<\/jats:italic>. We demonstrate that both CheY1 and CheY2 are phosphorylated by CheA\u223cP and that the three CheV proteins mediate the dephosphorylation of CheA\u223cP, but with a clearly reduced efficiency as compared to CheY1 and CheY2. Furthermore, our data indicate retrophosphorylation of CheAY2 by CheY1\u223cP, suggesting a role of CheY2 as a phosphate sink to modulate the half-life of CheY1\u223cP.<\/jats:p>","DOI":"10.1099\/mic.0.28217-0","type":"journal-article","created":{"date-parts":[[2005,10,5]],"date-time":"2005-10-05T22:08:00Z","timestamp":1128550080000},"page":"3299-3311","source":"Crossref","is-referenced-by-count":63,"title":["Phosphate flow in the chemotactic response system of Helicobacter pylori"],"prefix":"10.1099","volume":"151","author":[{"given":"Mar\u00eda-Antonieta","family":"Jim\u00e9nez-Pearson","sequence":"first","affiliation":[{"name":"Theodor-Boveri-Institut f\u00fcr Biowissenschaften, Lehrstuhl f\u00fcr Mikrobiologie, Universit\u00e4t W\u00fcrzburg, Am Hubland, D-97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Delany","sequence":"additional","affiliation":[{"name":"Molecular Immunology Unit, Chiron Vaccines, Via Fiorentina 1, 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