{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:36:28Z","timestamp":1791311788729,"version":"4.3.1"},"reference-count":62,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,1]]},"abstract":"<jats:p>Group B streptococci (GBS) are pathogens of both neonates and adults, with serotype III strains in particular being associated with invasive disease and meningitis. In this study, a novel GBS surface antigen, <jats:italic>\u03b5<\/jats:italic>, was found to be co-expressed with the previously reported <jats:italic>\u03b4<\/jats:italic> antigen on an identical subset of serotype III GBS. Expression of <jats:italic>\u03b4<\/jats:italic>\/<jats:italic>\u03b5<\/jats:italic> on the surface of serotype III GBS was shown to distinguish the restriction digest pattern (RDP) III-3 and multilocus sequence typing (ST)-17 lineage. <jats:italic>\u03b5<\/jats:italic>-Specific antibodies were reactive with a unique, high-molecular-mass, serine-rich repeat protein (Srr-2) found exclusively in RDP III-3 strains. The gene encoding Srr-2 was located within a putative accessory secretory locus that included <jats:italic>secY2<\/jats:italic> and <jats:italic>secA2<\/jats:italic> homologues and had a genetic organization similar to that of the <jats:italic>secY2<\/jats:italic>\/<jats:italic>A2<\/jats:italic> locus of staphylococci. In contrast, serotype III <jats:italic>\u03b4<\/jats:italic>\/<jats:italic>\u03b5<\/jats:italic>-negative strains and strains representative of serotypes Ia, Ib, Ic and II shared a common Srr-encoding gene, <jats:italic>srr-1<\/jats:italic>, and an organization of the <jats:italic>secY2<\/jats:italic>\/<jats:italic>A2<\/jats:italic> locus similar to that of previously reported serotype Ic, <jats:italic>\u03b4<\/jats:italic>\/<jats:italic>\u03b5<\/jats:italic>-negative serotype III and serotype V GBS strains. Representative serotype III <jats:italic>\u03b4<\/jats:italic>\/<jats:italic>\u03b5<\/jats:italic>-positive strains had LD<jats:sub>90<\/jats:sub> values 3\u20134 logs less than those of serotype III <jats:italic>\u03b4<\/jats:italic>\/<jats:italic>\u03b5<\/jats:italic>-negative strains in a neonatal mouse model of infection. These results indicate that the RDP III-3\/ST-17 lineage expresses Srr-2 and is highly virulent in an <jats:italic>in vivo<\/jats:italic> model of neonatal sepsis.<\/jats:p>","DOI":"10.1099\/mic.0.28516-0","type":"journal-article","created":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T21:18:23Z","timestamp":1142889503000},"page":"1029-1040","source":"Crossref","is-referenced-by-count":112,"title":["A unique serine-rich repeat protein (Srr-2) and novel surface antigen (\u03b5) associated with a virulent lineage of serotype III Streptococcus agalactiae"],"prefix":"10.1099","volume":"152","author":[{"given":"Kyle N.","family":"Seifert","sequence":"first","affiliation":[{"name":"Department of Biology, James Madison University, Harrisonburg, VA 22807, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth E.","family":"Adderson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April A.","family":"Whiting","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Bohnsack","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula J.","family":"Crowley","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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