{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:24:28Z","timestamp":1790965468361,"version":"4.1.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,6,1]]},"abstract":"<jats:p>The complete structures of LPS core types 1 and 2 from<jats:italic>Klebsiella pneumoniae<\/jats:italic>have been described by other authors. They are characterized by a lack of phosphoryl residues, but they contain galacturonic acid (GalA) residues, which contribute to the necessary negative charges. The presence of a capsule was determined in core-LPS non-polar mutants from strains 52145 (O1\u200a:\u200aK2), DL1 (O1\u200a:\u200aK1) and C3 (O8\u200a:\u200aK66). O-antigen ligase (<jats:italic>waaL<\/jats:italic>) mutants produced a capsule. Core mutants containing the GalA residues were capsulated, while those lacking the residues were non capsulated. Since the proteins involved in the transfer of GalA (WabG) and glucosamine residues (WabH) are known, the chemical basis of the capsular-K2\u2013cell-surface association was studied. Phenol\/water extracts from<jats:italic>K. pneumoniae<\/jats:italic>52145\u0394<jats:italic>wabH waaL<\/jats:italic>and 52145\u0394<jats:italic>waaL<\/jats:italic>mutants, but not those from from<jats:italic>K. pneumoniae<\/jats:italic>52145\u0394<jats:italic>wabG waaL<\/jats:italic>mutant, contained both LPS and capsular polysaccharide, even after hydrophobic chromatography. The two polysaccharides were dissociated by gel-filtration chromatography, eluting with detergent and metal-ion chelators. From these results, it is concluded that the K2 capsular polysaccharide is associated by an ionic interaction to the LPS through the negative charge provided by the carboxyl groups of the GalA residues.<\/jats:p>","DOI":"10.1099\/mic.0.28611-0","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:40:51Z","timestamp":1149064851000},"page":"1807-1818","source":"Crossref","is-referenced-by-count":54,"title":["The ionic interaction of Klebsiella pneumoniae K2 capsule and core lipopolysaccharide"],"prefix":"10.1099","volume":"152","author":[{"given":"Sandra","family":"Fresno","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Merino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Michela","family":"Corsaro","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica e Biochimica, Universit\u00e0 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"De Castro","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica e Biochimica, Universit\u00e0 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelangelo","family":"Parrilli","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica e Biochimica, Universit\u00e0 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Naldi","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica e Biochimica, Universit\u00e0 Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Regu\u00e9","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s\/n, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Tom\u00e1s","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/IAI.63.3.903-910.1995","article-title":"A porin from Klebsiella pneumoniae : sequence homology, three-dimensional model, and complement binding","volume":"63","author":"Alberti\u0301","year":"1995","journal-title":"Infect Immun"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1128\/JB.177.7.1788-1796.1995","article-title":"Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in virulent strain Chedid","volume":"177","author":"Arakawa","year":"1995","journal-title":"J Bacteriol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0882-4010(92)90074-X","article-title":"Modulation of surface antigen expression by Klebsiella pneumoniae in response to growth environment","volume":"13","author":"Camprubi\u0301","year":"1992","journal-title":"Microb Pathog"},{"key":"R4","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0923-2508(97)87644-9","article-title":"Molecular characterization of a 17-kDa outer-membrane protein from Klebsiella pneumoniae","volume":"148","author":"Climent","year":"1997","journal-title":"Res Microbiol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1016\/j.carres.2005.07.006","article-title":"[sup]1[\/sup]H and [sup]13[\/sup]C-NMR characterisation and secondary structure of the K2 polysaccharide of Klebsiella pneumoniae strain 52145","volume":"340","author":"Corsaro","year":"2005","journal-title":"Carbohydr Res"},{"key":"R6","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1128\/IAI.70.5.2583-2590.2002","article-title":"Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia","volume":"70","author":"Corte\u0301s","year":"2002","journal-title":"Infect Immun"},{"key":"R7","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1128\/CMR.6.4.428","article-title":"An overview of nosocomial infections, including the role of the microbiology laboratory","volume":"6","author":"Emori","year":"1993","journal-title":"Clin Microbiol Rev"},{"key":"R8","doi-asserted-by":"crossref","first-page":"27928","DOI":"10.1074\/jbc.M402549200","article-title":"Biosynthesis of a novel 3-deoxy-d- manno -oct-ulosonic acid-containing outer core oligosaccharide in the lipopolysaccharide of Klebsiella pneumoniae","volume":"279","author":"Frirdich","year":"2004","journal-title":"J Biol Chem"},{"key":"R9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1432-1033.1969.tb00601.x","article-title":"A new method for the extraction of R lipopolysaccharides","volume":"9","author":"Galanos","year":"1969","journal-title":"Eur J Biochem"},{"key":"R10","doi-asserted-by":"crossref","first-page":"8915","DOI":"10.1016\/S0021-9258(19)52486-3","article-title":"Lipid on capsular polysaccharides of Gram-negative bacteria","volume":"256","author":"Gotschlich","year":"1981","journal-title":"J Biol Chem"},{"key":"R11","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1128\/JCM.37.1.56-62.1999","article-title":"Klebsiella pneumoniae lipopolysaccharide O typing: revision of prototype strains and O-group distribution among clinical isolates from different sources and countries","volume":"37","author":"Hansen","year":"1999","journal-title":"J Clin Microbiol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/j.1432-1033.1996.0272n.x","article-title":"Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3","volume":"237","author":"Helander","year":"1996","journal-title":"Eur J Biochem"},{"key":"R13","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1099\/13500872-145-3-673","article-title":"Porin expression in clinical isolates of Klebsiella pneumoniae","volume":"145","author":"Herna\u0301ndez-Alle\u0301s","year":"1999","journal-title":"Microbiology"},{"key":"R14","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/clind\/16.5.719","article-title":"Neonatal sepsis and meningitis in Mallorca (Spain), 1977\u20131991","volume":"16","author":"Herva\u0301s","year":"1993","journal-title":"Clin Infect Dis"},{"key":"R15","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/JB.154.1.269-277.1983","article-title":"Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels","volume":"154","author":"Hitchcock","year":"1983","journal-title":"J Bacteriol"},{"key":"R16","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1128\/JB.173.6.1862-1866.1991","article-title":"Structure, serological specificity, and synthesis of artificial glycoconjugates representing the genus-specific lipopolysaccharide epitope of Chlamydia spp","volume":"173","author":"Holst","year":"1991","journal-title":"J Bacteriol"},{"key":"R17","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1111\/j.1432-1033.1993.tb17971.x","article-title":"Structural analysis of two oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus-specific epitope of Chlamydia lipopolysaccharide","volume":"214","author":"Holst","year":"1993","journal-title":"Eur J Biochem"},{"key":"R18","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.1099\/00221287-148-11-3485","article-title":"The inner-core lipopolysaccharide biosynthetic waaE gene: function and genetic distribution among some Enterobacteriaceae","volume":"148","author":"Izquierdo","year":"2002","journal-title":"Microbiology"},{"key":"R19","doi-asserted-by":"crossref","first-page":"7213","DOI":"10.1128\/JB.185.24.7213-7221.2003","article-title":"The Klebsiella pneumoniae wabG gene: its role in the biosynthesis of the core lipopolysaccharide and virulence","volume":"185","author":"Izquierdo","year":"2003","journal-title":"J Bacteriol"},{"key":"R20","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/S0934-8840(11)80006-X","article-title":"Core-lipid A on the K40 polysaccharide of Escherichia coli O8\u200a:\u200aK40\u200a:\u200aH9, a representative of group I capsular polysaccharides","volume":"276","author":"Jann","year":"1992","journal-title":"Zentralbl Bakteriol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1365-2958.1993.tb00933.x","article-title":"Structural variation in the O-specific polysaccharides of Klebsiella pneumoniae serotype O1 and O8 lipopolysaccharide: evidence for clonal diversity in rfb genes","volume":"10","author":"Kelly","year":"1993","journal-title":"Mol Microbiol"},{"key":"R22","first-page":"1325","article-title":"The structures of lipopolysaccharides of Gram-negative bacteria. III. The structure of O-antigens: a review","volume":"59","author":"Knirel","year":"1994","journal-title":"Biochemistry"},{"key":"R23","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1128\/JB.163.2.769-773.1985","article-title":"Evidence for covalent attachment of phospholipid to the capsular polysaccharide of Haemophilus influenzae type b","volume":"163","author":"Kuo","year":"1985","journal-title":"J Bacteriol"},{"key":"R24","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1128\/JB.185.3.788-800.2003","article-title":"RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level","volume":"185","author":"Lai","year":"2003","journal-title":"J Bacteriol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"6228","DOI":"10.1128\/JB.179.20.6228-6237.1997","article-title":"Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli : application to open reading frame characterization","volume":"179","author":"Link","year":"1997","journal-title":"J Bacteriol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1128\/AAC.40.2.342","article-title":"In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum-cephalosporins","volume":"40","author":"Marti\u0301nez-Marti\u0301nez","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"R27","volume-title":"Experiments in Molecular Genetics","author":"Miller","year":"1972"},{"key":"R28","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0378-4347(99)00256-X","article-title":"Efficient method of highly purified lipopolysaccharides by hydrophobic interaction chromatography","volume":"732","author":"Muck","year":"1999","journal-title":"J Chromatogr B Biomed Sci Appl"},{"key":"R29","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1128\/IAI.57.2.546-552.1989","article-title":"Mucoid phenotype of Klebsiella pneumoniae is a plasmid-encoded virulence factor","volume":"57","author":"Nassif","year":"1989","journal-title":"Infect Immun"},{"key":"R30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/MMBR.49.1.1-32.1985","article-title":"Molecular basis of bacterial outer membrane permeability","volume":"49","author":"Nikaido","year":"1985","journal-title":"Microbiol Rev"},{"key":"R31","first-page":"143","article-title":"Serotyping of Klebsiella","volume-title":"Methods in Microbiology","author":"\u00d8rskov","year":"1984"},{"key":"R32","doi-asserted-by":"crossref","first-page":"6428","DOI":"10.1128\/JB.173.20.6428-6431.1991","article-title":"Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12","volume":"173","author":"Pradel","year":"1991","journal-title":"J Bacteriol"},{"key":"R33","doi-asserted-by":"crossref","first-page":"5882","DOI":"10.1128\/JB.185.19.5882-5890.2003","article-title":"A novel outer membrane protein, Wzi, is involved in surface assembly of the Escherichia coli K30 group 1 capsule","volume":"185","author":"Rahn","year":"2003","journal-title":"J Bacteriol"},{"key":"R34","doi-asserted-by":"crossref","first-page":"3564","DOI":"10.1128\/JB.183.12.3564-3573.2001","article-title":"Genetic characterization of the Klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis","volume":"183","author":"Regue\u0301","year":"2001","journal-title":"J Bacteriol"},{"key":"R35","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1128\/IAI.72.1.54-61.2004","article-title":"A gene (uridinediphosphate galacturonate 4-epimerase) is essential for Klebsiella pneumoniae virulence","volume":"72","author":"Regue\u0301","year":"2004","journal-title":"Infect Immun"},{"key":"R36","doi-asserted-by":"crossref","first-page":"36648","DOI":"10.1074\/jbc.M506278200","article-title":"The incorporations of glucosamine to enterobacterial core LPS: two enzymatic steps are required","volume":"280","author":"Regue\u0301","year":"2005a","journal-title":"J Biol Chem"},{"key":"R37","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1128\/JB.187.12.4198-4206.2005","article-title":"A second outer-core region in Klebsiella pneumoniae lipopolysaccharide","volume":"187","author":"Regue\u0301","year":"2005b","journal-title":"J Bacteriol"},{"key":"R38","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"R39","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1128\/IAI.50.3.787-795.1985","article-title":"Importance of a lipopolysaccharide-containing extracellular toxic complex in infections produced by Klebsiella pneumoniae","volume":"50","author":"Straus","year":"1985","journal-title":"Infect Immun"},{"key":"R40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0008-6215(95)00002-B","article-title":"Structural investigation on the carbohydrate backbone of the lipopolysaccharide from Klebsiella pneumoniae rough mutant R20\/O1[sup]\u2212[\/sup]","volume":"269","author":"Su\u0308sskind","year":"1995","journal-title":"Carbohydr Res"},{"key":"R41","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0882-4010(88)90016-2","article-title":"Surface exposure of the O-antigen in Klebsiella pneumoniae O1\u200a:\u200aK1 serotype strains","volume":"5","author":"Toma\u0301s","year":"1988","journal-title":"Microb Pathog"},{"key":"R42","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","article-title":"A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels","volume":"119","author":"Tsai","year":"1982","journal-title":"Anal Biochem"},{"key":"R43","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0008-6215(01)00216-6","article-title":"Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae","volume":"335","author":"Vinogradov","year":"2001","journal-title":"Carbohydr Res"},{"key":"R44","doi-asserted-by":"crossref","first-page":"25070","DOI":"10.1074\/jbc.M202683200","article-title":"Structures of lipopolysaccharides from Klebsiella pneumoniae . Eluicidation of the structure of the linkage region between core and polysaccharide O chain and identification of the residues at the non-reducing termini of the O chains","volume":"277","author":"Vinogradov","year":"2002","journal-title":"J Biol Chem"},{"key":"R45","first-page":"83","article-title":"Bacterial lipopolysaccharide extraction with phenol\u2013water and further application of the procedure","volume":"5","author":"Westphal","year":"1965","journal-title":"Methods Carbohydr Chem"},{"key":"R46","first-page":"196","article-title":"The pathogenicity of Klebsiella pneumoniae","volume":"1","author":"Williams","year":"1990","journal-title":"Rev Med Microbiol"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.28611-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T21:57:59Z","timestamp":1627423079000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.28611-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6,1]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.28611-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6,1]]}}}