{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:40:57Z","timestamp":1773657657695,"version":"3.50.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,1]]},"abstract":"<jats:p><jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>, the causative agent of swine enzootic pneumonia and a major component of the porcine respiratory disease complex, continues to confound swine producers despite control programmes worldwide. The disease is chronic and self-limiting, but the host is subject to immunopathological changes that potentiate respiratory disease associated with other pathogens. The response of<jats:italic>M. hyopneumoniae<\/jats:italic>to environmental stress is of interest because of its relevance to virulence mechanisms in other bacterial pathogens. One of these stressors, iron deprivation, is a prominent feature of the host innate immune response, and most certainly impacts growth of mycoplasmas<jats:italic>in vivo<\/jats:italic>. To study this, microarray technology was applied to the transcriptome analysis of<jats:italic>M. hyopneumoniae<\/jats:italic>during iron deprivation. An array consisting of 632 of the 698 ORFs in the genome was used to compare the mRNA isolated from organisms grown under normal laboratory conditions with that from organisms subjected to iron deprivation with the chelator 2,2\u2032-dipyridyl. This analysis identified 27 genes that were either up- or down-regulated in response to low-iron growth conditions (<jats:italic>P<\/jats:italic>&lt;0\u00b701), with an estimated false discovery rate below 10\u200a%. These included genes encoding transport proteins, enzymes involved in energy metabolism, and components of the translation process. Ten of the 27 identified genes had no assigned function. These studies indicate that<jats:italic>M. hyopneumoniae<\/jats:italic>can respond to changes in environmental conditions, but the mechanism employed remains unknown.<\/jats:p>","DOI":"10.1099\/mic.0.28674-0","type":"journal-article","created":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T21:18:23Z","timestamp":1142889503000},"page":"937-944","source":"Crossref","is-referenced-by-count":37,"title":["Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays"],"prefix":"10.1099","volume":"152","author":[{"given":"Melissa L.","family":"Madsen","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Nettleton","sequence":"additional","affiliation":[{"name":"Department of Statistics, Iowa State University, Ames, IA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen L.","family":"Thacker","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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