{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:06Z","timestamp":1790317206710,"version":"4.1.0"},"reference-count":53,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,1]]},"abstract":"<jats:p><jats:italic>Sphingomonas xenophaga<\/jats:italic>BN6 degrades various (substituted) naphthalenesulfonates to the corresponding (substituted) salicylates. A gene cluster was identified on the plasmid pBN6 which coded for several enzymes participating in the degradative pathway for naphthalenesulfonates. A DNA fragment of 16\u200a915\u2005bp was sequenced which contained 17 ORFs. The genes encoding the 1,2-dihydroxynaphthalene dioxygenase, 2-hydroxychromene-2-carboxylate isomerase, and 2\u2032-hydroxybenzalpyruvate aldolase of the naphthalenesulfonate pathway were identified on the DNA fragment and the encoded proteins heterologously expressed in<jats:italic>Escherichia coli<\/jats:italic>. Also, the genes encoding the ferredoxin and ferredoxin reductase of a multi-component, ring-hydroxylating naphthalenesulfonate dioxygenase were identified by insertional inactivation. The identified genes generally demonstrated the highest degree of homology to enzymes encoded by the phenanthrene-degrading organism<jats:italic>Sphingomonas<\/jats:italic>sp. P2, or the megaplasmid pNL1 of the naphthalene- and biphenyl-degrading strain<jats:italic>Sphingomonas aromaticivorans<\/jats:italic>F199. The genes of<jats:italic>S. xenophaga<\/jats:italic>BN6 participating in the degradation of naphthalenesulfonates also shared the same organization in three different transcriptional units as the genes involved in the degradation of naphthalene, biphenyl, and phenanthrene previously found in<jats:italic>Sphingomonas<\/jats:italic>sp. P2 and<jats:italic>S. aromaticivorans<\/jats:italic>F199. The genes were flanked in<jats:italic>S. xenophaga<\/jats:italic>BN6 by ORFs which specify proteins that show the highest homologies to proteins of mobile genetic elements.<\/jats:p>","DOI":"10.1099\/mic.0.28783-0","type":"journal-article","created":{"date-parts":[[2006,6,27]],"date-time":"2006-06-27T23:04:33Z","timestamp":1151449473000},"page":"1929-1940","source":"Crossref","is-referenced-by-count":26,"title":["Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6"],"prefix":"10.1099","volume":"152","author":[{"given":"Andreas","family":"Keck","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Industrielle Genetik, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Conradt","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Industrielle Genetik, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Mahler","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Industrielle Genetik, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Stolz","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Mattes","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Industrielle Genetik, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Klein","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Industrielle Genetik, Universit\u00e4t Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1021\/ac981377e","article-title":"Solid-phase extraction procedure of polar benzene- and naphthalenesulfonates in industrial effluents followed by unequivocal determination with ion-pair chromatography\/electrospray-mass spectrometry","volume":"71","author":"Alonso","year":"1999","journal-title":"Anal Chem"},{"key":"R2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0378-1119(93)90540-J","article-title":"A new \u03bb RES vector with a built-in Tn 1721 -encoded excision system","volume":"123","author":"Altenbuchner","year":"1993","journal-title":"Gene"},{"key":"R3","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/0076-6879(92)16042-I","article-title":"Positive selection vectors based on palindromic DNA sequences","volume":"216","author":"Altenbuchner","year":"1992","journal-title":"Methods Enzymol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"3862","DOI":"10.1128\/JB.186.12.3862-3872.2004","article-title":"Detection and characterization of conjugative degradative plasmids in xenobiotics-degrading Sphingomonas strains","volume":"186","author":"Basta","year":"2004","journal-title":"J Bacteriol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1099\/mic.0.27965-0","article-title":"Structural and replicative diversity of large plasmids from sphingomonads that degrade polycyclic aromatic compounds and xenobiotics","volume":"151","author":"Basta","year":"2005","journal-title":"Microbiology"},{"key":"R7","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"R8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1128\/AEM.42.1.44-55.1981","article-title":"Catabolism of naphthalenesulfonic acids by Pseudomonas sp.A3 and Pseudomonas sp. 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