{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:15:00Z","timestamp":1789996500421,"version":"4.0.1"},"reference-count":68,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,1]]},"abstract":"<jats:p>A genetic locus encoding erythritol uptake and catabolism genes was identified in <jats:italic>Rhizobium leguminosarum<\/jats:italic> bv. <jats:italic>viciae<\/jats:italic>, and shown to be plasmid encoded in a wide range of <jats:italic>R. leguminosarum<\/jats:italic> strains. A Tn<jats:italic>5<\/jats:italic>-B22 mutant (19B-3) unable to grow on erythritol was isolated from a mutant library of <jats:italic>R. leguminosarum<\/jats:italic> strain VF39SM. The mutated gene <jats:italic>eryF<\/jats:italic> was cloned and partially sequenced, and determined to have a high homology to permease genes of ABC transporters. A cosmid complementing the mutation (pCos42) was identified and was shown to carry all the genes necessary to restore the ability to grow on erythritol to a VF39SM strain cured of pRleVF39f. In the genomic DNA sequence of strain 3841, the gene linked to the mutation in 19B-3 is flanked by a cluster of genes with high homology to the known erythritol catabolic genes from <jats:italic>Brucella<\/jats:italic> spp. Through mutagenesis studies, three distinct operons on pCos42 that are required for growth on erythritol were identified: an ABC-transporter operon (<jats:italic>eryEFG<\/jats:italic>), a catabolic operon (<jats:italic>eryABCD<\/jats:italic>) and an operon (<jats:italic>deoR-tpiA2-rpiB<\/jats:italic>) that encodes a gene with significant homology to triosephosphate isomerase (<jats:italic>tpiA2<\/jats:italic>). These genes all share high sequence identity to genes in the erythritol catabolism region of <jats:italic>Brucella<\/jats:italic> spp., and <jats:sc>clustalw<\/jats:sc> alignments suggest that horizontal transfer of the erythritol locus may have occurred between <jats:italic>R. leguminosarum<\/jats:italic> and <jats:italic>Brucella<\/jats:italic>. Transcription of the <jats:italic>eryABCD<\/jats:italic> operon is repressed by EryD and is induced by the presence of erythritol. Mutant 19B-3 was impaired in its ability to compete against wild-type for nodulation of pea plants but was still capable of forming nitrogen-fixing nodules.<\/jats:p>","DOI":"10.1099\/mic.0.28938-0","type":"journal-article","created":{"date-parts":[[2006,6,27]],"date-time":"2006-06-27T23:04:33Z","timestamp":1151449473000},"page":"2061-2074","source":"Crossref","is-referenced-by-count":65,"title":["Characterization of genes involved in erythritol catabolism in Rhizobium leguminosarum bv. viciae"],"prefix":"10.1099","volume":"152","author":[{"given":"Christopher K.","family":"Yost","sequence":"first","affiliation":[{"name":"Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, SK S4S 0A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber M.","family":"Rath","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Calgary, 2500 University Drive, Calgary, AB T2N 1N4, 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