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Two genes from <jats:italic>Bifidobacterium breve<\/jats:italic> encoding hypothetical membrane proteins with a high homology with members of the ATP-binding cassette (ABC) family of multidrug efflux transporters, were expressed separately and jointly in <jats:italic>Lactococcus lactis<\/jats:italic>. Cells co-expressing both proteins exhibited enhanced resistance levels to the antimicrobials nisin and polymyxin B. Furthermore, the drug extrusion activity in membrane vesicles was increased when both proteins were co-expressed, compared to membranes in which the proteins were produced independently. Both proteins were co-purified from the membrane as a stable complex in a 1\u200a:\u200a1 ratio. This is believed to be the first study of a functional ABC-type multidrug transporter in <jats:italic>Bifidobacterium<\/jats:italic> and contributes to our understanding of the molecular mechanisms underlying the capacity of intestinal bacteria to tolerate cytotoxic compounds.<\/jats:p>","DOI":"10.1099\/mic.0.29097-0","type":"journal-article","created":{"date-parts":[[2006,12,12]],"date-time":"2006-12-12T08:28:10Z","timestamp":1165912090000},"page":"3497-3505","source":"Crossref","is-referenced-by-count":36,"title":["Two membrane proteins from Bifidobacterium breve UCC2003 constitute an ABC-type multidrug transporter"],"prefix":"10.1099","volume":"152","author":[{"given":"Abelardo","family":"Margolles","sequence":"first","affiliation":[{"name":"Instituto de Productos L\u00e1cteos de Asturias, Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Ctra Infiesto s\/n, 33300, Villaviciosa, Asturias, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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