{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:28:14Z","timestamp":1789388894731,"version":"build-2803163510"},"reference-count":41,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"The compound pyridine-2,6-bis(thiocarboxylic acid) (PDTC) is known to be produced and excreted by three strains of Pseudomonas<\/jats:italic>. Its reactivity includes the complete dechlorination of the environmental contaminant carbon tetrachloride. PDTC functions as a siderophore; however, roles as a ferric reductant and antimicrobial agent have also been proposed. PDTC function and regulation were further explored by characterizing the phenotypes of mutants in predicted membrane transporter genes. The functions of a predicted outer-membrane transporter (PdtK) and a predicted inner-membrane permease (PdtE) were examined in Pseudomonas putida<\/jats:italic> DSM 3601. Uptake of iron from 55<\/jats:sup>Fe(III):PDTC, and bioutilization of PDTC in a chelated medium, were dependent upon PdtK and PdtE. Another strain of P. putida<\/jats:italic> (KT2440), which lacks pdt<\/jats:italic> orthologues, showed growth inhibition by PDTC that could be relieved by introducing a plasmid containing pdt<\/jats:italic>KCPE. Transcriptional activation in response to exogenously added PDTC (25\u2005\u03bcM) was unaltered by the pdt<\/jats:italic>K or pdt<\/jats:italic>E mutations; each mutant showed activation of a pdt<\/jats:italic> transcriptional reporter, indistinguishable from an isogenic PDTC utilization-proficient strain. The data demonstrate that PdtK and PdtE constitute a bipartite outer-membrane\/inner-membrane transport system for iron acquisition from Fe(III):PDTC. Disruptions in this portion of the P. putida<\/jats:italic> DSM 3601 pdt<\/jats:italic> gene cluster do not abolish PDTC-dependent transcriptional signalling.<\/jats:p>","DOI":"10.1099\/mic.0.29116-0","type":"journal-article","created":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T23:08:18Z","timestamp":1159398498000},"page":"3157-3166","source":"Crossref","is-referenced-by-count":13,"title":["Identification and characterization of Pseudomonas membrane transporters necessary for utilization of the siderophore pyridine-2,6-bis(thiocarboxylic acid) (PDTC)"],"prefix":"10.1099","volume":"152","author":[{"given":"Lynne H.","family":"Leach","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Vermont, 95 Carrigan Drive, Burlington, VT 05405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular 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