{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:25:05Z","timestamp":1791307505015,"version":"4.3.1"},"reference-count":47,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,12,1]]},"abstract":"<jats:p>Despite accumulating data implicating<jats:italic>Propionibacterium acnes<\/jats:italic>in a variety of diseases, its precise role in infection remains to be determined.<jats:italic>P. acnes<\/jats:italic>antigen-specific CD4<jats:sup>+<\/jats:sup>T cells are present in early inflamed acne lesions and may be involved in the inflammatory response; however, little is known about the specific antigens involved. In this study, B cell and T cell antigens from<jats:italic>P. acnes<\/jats:italic>expression libraries were cloned and evaluated and the four predominant proteins identified were investigated. Two of these antigens share some homology with an M-like protein of<jats:italic>Streptococcus equi<\/jats:italic>and have dermatan-sulphate-binding activity (PA-25957 and 5541). The remaining two antigens, PA-21693 and 4687, are similar to the product of the<jats:italic>Corynebacterium diphtheriae htaA<\/jats:italic>gene from the<jats:italic>hmu<\/jats:italic>ABC transport locus, although only one of these (PA-21693) is encoded within an<jats:italic>hmu<\/jats:italic>-like operon and conserved amongst a range of clinical isolates. All four proteins contain an LPXTG motif, although only PA-21693 contains a characteristic sortase-sorting signal. Variation in the expression of PA-4687, 25957 and 5541 is evident amongst clinical isolates and is generated both by frameshifts associated with the putative signal peptide and by variable numbers of repeat regions toward the carboxy-terminus, potentially generating heterogeneity of molecular mass and antigenic variation. In addition, in the case of PA-25957, a frameshift in a C-rich region at the extreme carboxy-terminus eliminates the LPXTG motif in some isolates. For the dermatan-sulphate-binding PA-25957, IgG1 antibody in serum from acne-positive donors was shown to be specific for the amino-terminal region of the protein, which also contains a CD4<jats:sup>+<\/jats:sup>T cell epitope. In contrast, serum from acne-negative donors shows an IgG2 and IgG3 antibody subclass response to the carboxy-terminal region. These data have implications for the potential role of<jats:italic>P. acnes<\/jats:italic>in inflammatory acne and other diseases.<\/jats:p>","DOI":"10.1099\/mic.0.29219-0","type":"journal-article","created":{"date-parts":[[2006,12,12]],"date-time":"2006-12-12T08:28:10Z","timestamp":1165912090000},"page":"3667-3681","source":"Crossref","is-referenced-by-count":65,"title":["Variable expression of immunoreactive surface proteins of Propionibacterium acnes"],"prefix":"10.1099","volume":"152","author":[{"given":"Michael J.","family":"Lodes","sequence":"first","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Secrist","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darin R.","family":"Benson","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyian","family":"Jen","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt D.","family":"Shanebeck","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Guderian","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Maisonneuve","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Persing","sequence":"additional","affiliation":[{"name":"Corixa Corporation, Infectious Disease Research Institute, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"Patrick","sequence":"additional","affiliation":[{"name":"Microbiology Building, School of Medicine and Dentistry, Queen's University Belfast, Grosvenor Road, Belfast BT12 6BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasir A. 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