{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:50:59Z","timestamp":1779904259893,"version":"3.53.1"},"reference-count":35,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:p>Several instances of emerging diseases in humans appear to be caused by the spillover of viruses endemic to bats, either directly or through other animal intermediaries. The objective of this study was to detect, identify and characterize viruses in bats in the province of Manitoba and other regions of Canada. Bats were sampled from three sources: live-trapped <jats:italic>Myotis lucifugus<\/jats:italic> from Manitoba, rabies-negative <jats:italic>Eptesicus fuscus<\/jats:italic>, <jats:italic>M. lucifugus<\/jats:italic>, <jats:italic>M. yumanensis<\/jats:italic>, <jats:italic>M. septentrionalis<\/jats:italic>, <jats:italic>M. californicus<\/jats:italic>, <jats:italic>M. evotis<\/jats:italic>, <jats:italic>Lasionycteris<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>noctivagans<\/jats:italic> and <jats:italic>Lasiurus<\/jats:italic> (<jats:italic>Las.<\/jats:italic>) <jats:italic>cinereus<\/jats:italic>, provided by the Centre of Expertise for Rabies of the Canadian Food Inspection Agency (CFIA), and <jats:italic>L. noctivagans<\/jats:italic>, <jats:italic>Las. cinereus and Las. borealis<\/jats:italic> collected from a wind farm in Manitoba. We attempted to isolate viruses from fresh tissue samples taken from trapped bats in cultured cells of bat, primate, rodent, porcine, ovine and avian origin. We also screened bat tissues by PCR using primers designed to amplify nucleic acids from members of certain families of viruses. We detected RNA of a group 1 coronavirus from <jats:italic>M. lucifugus<\/jats:italic> (3 of 31 animals) and DNA from an as-yet undescribed polyomavirus from female <jats:italic>M. lucifugus<\/jats:italic> (4 of 31 animals) and <jats:italic>M. californicus<\/jats:italic> (pooled tissues from two females).<\/jats:p>","DOI":"10.1099\/vir.0.010694-0","type":"journal-article","created":{"date-parts":[[2009,7,10]],"date-time":"2009-07-10T18:38:34Z","timestamp":1247251114000},"page":"2015-2022","source":"Crossref","is-referenced-by-count":82,"title":["Detection of polyoma and corona viruses in bats of Canada"],"prefix":"10.1099","volume":"90","author":[{"given":"Vikram","family":"Misra","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, 52 Campus Road, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Dumonceaux","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Dubois","sequence":"additional","affiliation":[{"name":"Manitoba Conservation, Wildlife and Ecosystem Protection Branch, Box 24, 200 Saulteaux Crescent, Winnipeg, MB R3J 3W3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Willis","sequence":"additional","affiliation":[{"name":"Department of Biology and Centre for Forest Interdisciplinary Research, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB R3B 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Nadin-Davis","sequence":"additional","affiliation":[{"name":"Centre of Expertise for Rabies, Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Severini","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Wandeler","sequence":"additional","affiliation":[{"name":"Centre of Expertise for Rabies, Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robbin","family":"Lindsay","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Disease, 1015 Arlington Street, Winnipeg, MB R3E 3R2, 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