{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:55:41Z","timestamp":1784681741316,"version":"3.55.0"},"reference-count":60,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,6,1]]},"abstract":"<jats:p>Japanese encephalitis virus (JEV) genome is a single-stranded, positive-sense RNA with non-coding regions (NCRs) of 95 and 585\u2005bases at its 5\u2032 and 3\u2032 ends, respectively. These may bind to viral or host proteins important for viral replication. It has been shown previously that three proteins of 32, 35 and 50\u2005kDa bind the 3\u2032 stem\u2013loop (SL) structure of the JEV 3\u2032 NCR, and one of these was identified as 36\u2005kDa Mov34 protein. Using electrophoretic mobility-shift and UV cross-linking assays, as well as a yeast three-hybrid system, it was shown here that La protein binds to the 3\u2032 SL of JEV. The binding was stable under high-salt conditions (300\u2005mM KCl) and the affinity of the RNA\u2013protein interaction was high; the dissociation constant (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) for binding of La protein to the 3\u2032 SL was 12\u2005nM, indicating that this RNA\u2013protein interaction is physiologically plausible. Only the N-terminal half of La protein containing RNA recognition motifs 1 and 2 interacted with JEV RNA. An RNA toe-printing assay followed by deletion mutagenesis showed that La protein bound to predicted loop structures in the 3\u2032 SL RNA. Furthermore, it was shown that small interfering RNA-mediated downregulation of La protein resulted in repression of JEV replication in cultured cells.<\/jats:p>","DOI":"10.1099\/vir.0.010850-0","type":"journal-article","created":{"date-parts":[[2009,5,18]],"date-time":"2009-05-18T18:28:53Z","timestamp":1242671333000},"page":"1343-1352","source":"Crossref","is-referenced-by-count":45,"title":["La protein binds the predicted loop structures in the 3\u2032 non-coding region of Japanese encephalitis virus genome: role in virus replication"],"prefix":"10.1099","volume":"90","author":[{"given":"Surender","family":"Vashist","sequence":"first","affiliation":[{"name":"National Institute of Immunology, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Anantpadma","sequence":"additional","affiliation":[{"name":"National Institute of Immunology, New Delhi 110067, 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