{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:16:58Z","timestamp":1791029818157,"version":"4.1.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:p>Infection by hepatitis B virus (HBV) and dietary exposure to aflatoxin B<jats:sub>1<\/jats:sub> (AFB<jats:sub>1<\/jats:sub>) are the main risk factors for the development of chronic liver disease and hepatocellular carcinoma (HCC). How these factors cooperate is still largely unknown. AFB<jats:sub>1<\/jats:sub> activation leads to DNA adduction and mutagenesis, with a specific mutation at codon 249 in <jats:italic>TP53<\/jats:italic> (p.R249S). So far, only limited studies have addressed the effects of AFB<jats:sub>1<\/jats:sub> on HBV replication. We have analysed the effects of both risk factors on p53 induction during HBV infection in HepaRG, a cell line with hepatocyte-like morphology that metabolizes AFB<jats:sub>1<\/jats:sub> and supports HBV infection. Exposure to AFB<jats:sub>1<\/jats:sub> up to 5 \u00b5M induced a downregulation of HBV replication after 48 h, as measured by a decrease in viral antigens in the culture medium (HBsAg, HBeAg and large envelope protein) and in intracellular levels of HBV transcripts, DNA and HBsAg. Conversely, HBV infection did not significantly modify AFB<jats:sub>1<\/jats:sub>-DNA adduct formation or repair as assessed by immunodot-blot assay, and the induction of p53 in response to AFB<jats:sub>1<\/jats:sub> was similar in infected and non-infected HepaRG cells. Overall, our results suggest that AFB<jats:sub>1<\/jats:sub> exposure decreases HBV replication, whereas DNA damage by AFB<jats:sub>1<\/jats:sub> and subsequent p53 induction is not affected by the presence of the virus. Thus, in HepaRG cell line, AFB<jats:sub>1<\/jats:sub> and HBV do not cooperate to increase DNA damage by AFB<jats:sub>1<\/jats:sub>. Further studies on the effects of both factors in a context of chronicity are needed to better understand synergistic effects.<\/jats:p>","DOI":"10.1099\/vir.0.032482-0","type":"journal-article","created":{"date-parts":[[2011,11,24]],"date-time":"2011-11-24T06:01:05Z","timestamp":1322114465000},"page":"640-650","source":"Crossref","is-referenced-by-count":27,"title":["Interactions between hepatitis B virus and aflatoxin B1: effects on p53 induction in HepaRG cells"],"prefix":"10.1099","volume":"93","author":[{"given":"Myriam","family":"Lereau","sequence":"first","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"},{"name":"INSERM U1052, 151 cours Albert Thomas, 69424 Lyon Cedex 03, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doriane","family":"Gouas","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Villar","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Besaratinia","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Beckman Research Institute, City of Hope Comprehensive Cancer Center, 1500 East Duarte Road, Duarte, CA 91010-3000, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Hautefeuille","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Berthillon","sequence":"additional","affiliation":[{"name":"INSERM U1052, 151 cours Albert Thomas, 69424 Lyon Cedex 03, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislaine","family":"Martel-Planche","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Nogueira da Costa","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Ortiz-Cuaran","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Hantz","sequence":"additional","affiliation":[{"name":"INSERM U1052, 151 cours Albert Thomas, 69424 Lyon Cedex 03, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd P.","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Beckman Research Institute, City of Hope Comprehensive Cancer Center, 1500 East Duarte Road, Duarte, CA 91010-3000, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Hainaut","sequence":"additional","affiliation":[{"name":"International Center for Research and Training, Hospital AC Camargo, Rua Pirapitingu\u00ed 204, S\u00e3o Paulo SP 01508 - 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