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Viral proteins were sparse in the Golgi complex and accumulated in the ER. Ultrastructural analysis revealed that long filamentous structures, rather than spherical SPs, were observed in the lumen of the ER as a result of the mutation. The production of infectious virions derived from infectious cDNA of TBEV was also reduced by mutations in the conserved region. Molecular modelling analysis suggested that the conserved region is important for the association of prM\u2013envelope protein heterodimers in the formation of a spike of immature virion. These results are the first demonstration that the conserved region in the prM protein is a molecular determinant for the flavivirus assembly process.<\/jats:p>","DOI":"10.1099\/vir.0.035964-0","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T01:38:19Z","timestamp":1317260299000},"page":"27-38","source":"Crossref","is-referenced-by-count":48,"title":["A conserved region in the prM protein is a critical determinant in the assembly of flavivirus particles"],"prefix":"10.1099","volume":"93","author":[{"given":"Kentaro","family":"Yoshii","sequence":"first","affiliation":[{"name":"Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, 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