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Here, we demonstrate that one of these mAbs (16NS1) cross-reacted with Japanese encephalitis virus (JEV) and exhibited protective activity against a lethal JEV infection. Overlapping peptide mapping analysis combined with site-specific mutations identified a novel epitope<jats:sup>116<\/jats:sup>KAWGKSILFA<jats:sup>125<\/jats:sup>and critical amino acid residues (<jats:sup>118<\/jats:sup>W and<jats:sup>122<\/jats:sup>I) for 16NS1 mAb binding. These results may facilitate the development of a broadly therapeutic mAb that lacks enhancing potential and\/or subunit-based vaccine against flaviviruses that target the NS1 protein.<\/jats:p>","DOI":"10.1099\/vir.0.036640-0","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T07:36:35Z","timestamp":1316072195000},"page":"20-26","source":"Crossref","is-referenced-by-count":20,"title":["A cross-protective mAb recognizes a novel epitope within the flavivirus NS1 protein"],"prefix":"10.1099","volume":"93","author":[{"given":"Tae Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Institute for Medical Science, Chonbuk National University Medical School, Chonju, Chonbuk 561-180, Republic of Korea"},{"name":"Department of Microbiology and Immunology, Chonbuk National University Medical School, Chonju, Chonbuk 561-180, Republic of 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