{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:10:49Z","timestamp":1773321049979,"version":"3.50.1"},"reference-count":44,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:p>Generation of an abnormal isoform of the prion protein (PrP<jats:sup>Sc<\/jats:sup>) is a key aspect of the propagation of prions. Elucidation of the intracellular localization of PrP<jats:sup>Sc<\/jats:sup>in prion-infected cells facilitates the understanding of the cellular mechanism of prion propagation. However, technical improvement in PrP<jats:sup>Sc<\/jats:sup>-specific detection is required for precise analysis. Here, we show that the mAb 132, which recognizes the region adjacent to the most amyloidogenic region of PrP, is useful for PrP<jats:sup>Sc<\/jats:sup>-specific detection by immunofluorescence assay in cells pre-treated with guanidine thiocyanate. Extensive analysis of the intracellular localization of PrP<jats:sup>Sc<\/jats:sup>in prion-infected cells using mAb 132 revealed the presence of PrP<jats:sup>Sc<\/jats:sup>throughout endocytic compartments. In particular, some of the granular PrP<jats:sup>Sc<\/jats:sup>signals that were clustered at peri-nuclear regions appeared to be localized in an endocytic recycling compartment through which exogenously loaded transferrin, shiga and cholera toxin B subunits were transported. The granular PrP<jats:sup>Sc<\/jats:sup>signals at peri-nuclear regions were dispersed to the peripheral regions including the plasma membrane during incubation at 20 \u00b0C, at which temperature transport from the plasma membrane to peri-nuclear regions was impaired. Conversely, dispersed PrP<jats:sup>Sc<\/jats:sup>signals appeared to return to peri-nuclear regions within 30 min during subsequent incubation at 37 \u00b0C, following which PrP<jats:sup>Sc<\/jats:sup>at peri-nuclear regions appeared to redisperse again to peripheral regions over the next 30 min incubation. These results suggest that PrP<jats:sup>Sc<\/jats:sup>is dynamically transported along with the membrane trafficking machinery of cells and that at least some PrP<jats:sup>Sc<\/jats:sup>circulates between peri-nuclear and peripheral regions including the plasma membrane via an endocytic recycling pathway.<\/jats:p>","DOI":"10.1099\/vir.0.037101-0","type":"journal-article","created":{"date-parts":[[2011,11,17]],"date-time":"2011-11-17T12:34:30Z","timestamp":1321533270000},"page":"668-680","source":"Crossref","is-referenced-by-count":47,"title":["Characterization of intracellular localization of PrPSc in prion-infected cells using a mAb that recognizes the region consisting of aa 119\u2013127 of mouse PrP"],"prefix":"10.1099","volume":"93","author":[{"given":"Takeshi","family":"Yamasaki","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, 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