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Here, we have used replicase\u2013EGFP fusion proteins and recombinant baculovirus expression to demonstrate that the HaSV replicase associates strongly with cellular membranes, including detergent-resistant membranes, and that this association is maintained through a novel membrane targeting domain within the C-terminal region of the RNA-dependent RNA polymerase domain. We show a similar subcellular localization and strong association with detergent-resistant membranes for the carmo-like replicase of another tetravirus, Providence virus, in replicating cells, suggesting a common site of replication for these two tetraviruses.<\/jats:p>","DOI":"10.1099\/vir.0.038992-0","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T00:29:44Z","timestamp":1335486584000},"page":"1706-1716","source":"Crossref","is-referenced-by-count":5,"title":["Membrane targeting of an alpha-like tetravirus replicase is directed by a region within the RNA-dependent RNA polymerase domain"],"prefix":"10.1099","volume":"93","author":[{"given":"James R.","family":"Short","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, PO Box 94, Grahamstown, 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary A.","family":"Dorrington","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, PO Box 94, Grahamstown, 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.012104-0"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068886"},{"key":"r3","first-page":"327","article-title":"Structural comparison of insect RNA viruses","volume-title":"Insect Virology","author":"Banerjee","year":"2010"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1999.4858"},{"key":"r6","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1242\/jcs.114.23.4143","article-title":"Complexes of tetraspanins with integrins: more than meets the eye","volume":"114","author":"Berditchevski","year":"2001","journal-title":"J Cell Sci"},{"key":"r7","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1016\/S0021-9258(18)53930-2","article-title":"Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel","volume":"268","author":"Blachly-Dyson","year":"1993","journal-title":"J Biol Chem"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2011(02)00103-9"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1006\/bcon.2000.0887"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008650200"},{"key":"r11","first-page":"283","article-title":"The tetraviruses","volume-title":"Insect Virology","author":"Dorrington","year":"2010"},{"key":"r12","first-page":"1091","article-title":"Tetraviridae","volume-title":"Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses","author":"Dorrington","year":"2011"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2075"},{"key":"r14","first-page":"264","article-title":"Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium","volume":"12","author":"Gey","year":"1952","journal-title":"Cancer Res"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01033-1"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1132"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9677"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30701.x"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-9-2197"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80061-1"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1038\/nri1548"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(62)90290-8"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8906"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.05.019"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2216"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.7.4382-4395.2005"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(03)00081-4"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1038\/35074539"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(02)00052-1"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.08.006"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-007-0376-5"},{"key":"r43","article-title":"An investigation into the replication biology of Helicoverpa armigera stunt virus. 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