{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:58:48Z","timestamp":1762505928784},"reference-count":37,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"<jats:p>Varicella-zoster virus (VZV) causes chickenpox and shingles. VZV is released from infected cells during natural infection, but remains highly cell-associated during experimental infection, and so most studies have utilized cell-associated infection models. We examined the impact of cell-free VZV infection of primary human foreskin fibroblasts (HFFs) on the receptor integrin \u03b16 (ITGA6). qPCR and flow cytometry demonstrated that both cell-free VZV and cell-free UV-inactivated VZV downregulated transcription and cell-surface protein expression of ITGA6. To establish whether ITGA6 altered VZV infection, VZV transcripts and nuclear DNA levels were measured in HFFs treated with ITGA6 blocking antibody before infection. ITGA6 blocking did not impair virus entry but did negatively impact VZV transcription, and this effect was virus specific as transcription of the related herpes simplex virus type 1 was not similarly inhibited. This study identifies modulation of ITGA6 during cell-free VZV infection, and provides the first evidence linking ITGA6 with post-entry productive VZV gene expression.<\/jats:p>","DOI":"10.1099\/vir.0.039917-0","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T13:52:43Z","timestamp":1337262763000},"page":"1725-1730","source":"Crossref","is-referenced-by-count":6,"title":["Inhibition of integrin \u03b16 expression by cell-free varicella-zoster virus"],"prefix":"10.1099","volume":"93","author":[{"given":"Joshua B.","family":"Bowles","sequence":"first","affiliation":[{"name":"Centre for Virus Research, Westmead Millennium Institute, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Steain","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology, University of Sydney, NSW, Australia"},{"name":"Centre for Virus Research, Westmead Millennium Institute, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry","family":"Slobedman","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology, University of Sydney, NSW, Australia"},{"name":"Centre for Virus Research, Westmead Millennium Institute, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"Abendroth","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology, University of Sydney, NSW, Australia"},{"name":"Centre for Virus Research, Westmead Millennium Institute, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.4.1900-1907.2000"},{"key":"r2","first-page":"2731","article-title":"Varicella-zoster virus","volume-title":"Fields Virology","author":"Arvin","year":"2001"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-161869"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002693"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0250169"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00081-09"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907508106"},{"key":"r8","first-page":"2707","article-title":"Varicella-zoster virus and its replication","volume-title":"Fields Virology","author":"Cohen","year":"2001"},{"key":"r9","first-page":"2774","article-title":"Varicella-zoster virus replication, pathogenesis and management","volume-title":"Fields Virology","author":"Cohen","year":"2007"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-9-1759"},{"key":"r11","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1128\/JVI.71.3.2449-2456.1997","article-title":"Identification of the \u03b16 integrin as a candidate receptor for papillomaviruses","volume":"71","author":"Evander","year":"1997","journal-title":"J Virol"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406821101"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.05.002"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014923108"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-43-1-15"},{"key":"r16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1128\/JVI.14.1.8-19.1974","article-title":"Regulation of herpesvirus macromolecular synthesis. 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