{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:08:24Z","timestamp":1790845704870,"version":"4.1.0"},"reference-count":38,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:p>Group and serocomplex cross-reactive epitopes have been identified in the envelope (E) protein of several flaviviruses and have proven critical in vaccine and diagnostic antigen development. Here, we performed site-directed mutagenesis across the E gene of a recombinant expression plasmid that encodes the Japanese encephalitis virus (JEV) premembrane (prM) and E proteins and produces JEV virus-like particles (VLPs). Mutations were introduced at I135 and E138 in domain I; W101, G104, G106 and L107 in domain II; and T305, E306, K312, A315, S329, S331, G332 and D389 in domain III. None of the mutant JEV VLPs demonstrated reduced activity to the five JEV type-specific mAbs tested. Substitutions at W101, especially W101G, reduced reactivity dramatically with all of the flavivirus group cross-reactive mAbs. The group and JEV serocomplex cross-reactive mAbs examined recognized five and six different overlapping epitopes, respectively. Among five group cross-reactive epitopes, amino acids located in domains I, II and III were involved in one, five and three epitopes, respectively. Recognition by six JEV serocomplex cross-reactive mAbs was reduced by amino acid substitutions in domains II and III. These results suggest that amino acid residues located in the fusion loop of E domain II are the most critical for recognition by group cross-reactive mAbs, followed by residues of domains III and I. The amino acid residues of both domains II and III of the E protein were shown to be important in the binding of JEV serocomplex cross-reactive mAbs.<\/jats:p>","DOI":"10.1099\/vir.0.040238-0","type":"journal-article","created":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T07:54:09Z","timestamp":1329378849000},"page":"1185-1192","source":"Crossref","is-referenced-by-count":43,"title":["Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein"],"prefix":"10.1099","volume":"93","author":[{"given":"Shyan-Song","family":"Chiou","sequence":"first","affiliation":[{"name":"Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chin","family":"Fan","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne D.","family":"Crill","sequence":"additional","affiliation":[{"name":"Arboviral Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruey-Yi","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Life Science, National Dong Hwa University, Hualien, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwong-Jen J.","family":"Chang","sequence":"additional","affiliation":[{"name":"Arboviral Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.24.13097-13100.2002"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.99.5.429"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.003245"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.9.4244-4252.2000"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.11.056"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.10.047"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00004-08"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.24.13975-13986.2004"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82640-0"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004991"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83648-0"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00206-4"},{"key":"r13","doi-asserted-by":"crossref","first-page":"548","DOI":"10.4269\/ajtmh.1982.31.548","article-title":"Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies","volume":"31","author":"Gentry","year":"1982","journal-title":"Am J Trop Med Hyg"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00606-08"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1126\/science.3277268"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00346-9"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01735-06"},{"key":"r18","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/JVI.45.1.124-132.1983","article-title":"Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies","volume":"45","author":"Kimura-Kuroda","year":"1983","journal-title":"J Virol"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-12-2663"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)61001-8"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00316-08"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5448.2333"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59002-9"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2439"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1038\/nm1144"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.11.013"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1038\/nature02165"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01125-06"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01732-06"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1038\/375291a0"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90323-9"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90509-6"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(90)90532-V"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9200"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00080-06"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00432-07"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.10.033"},{"key":"r38","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1093\/oxfordjournals.epirev.a036087","article-title":"The epidemiology of Japanese encephalitis: prospects for prevention","volume":"14","author":"Vaughn","year":"1992","journal-title":"Epidemiol Rev"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.040238-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T23:25:28Z","timestamp":1603754728000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.040238-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,1]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.040238-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,1]]}}}