{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:21:35Z","timestamp":1791048095092,"version":"4.1.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,10,1]]},"abstract":"<jats:p>Dynamin2 is a large GTPase that regulates vesicle trafficking, and the GTPase activity of dynamin2 is required for the multistep process of adenovirus infection. Activity of dynamin2 may be regulated by post-translational phosphorylation and<jats:italic>S<\/jats:italic>-nitrosylation modifications. In this study, we demonstrate a role for dynamin2<jats:italic>S<\/jats:italic>-nitrosylation in adenovirus infection of epithelial cells. We show that adenovirus serotype 5 (Ad5) infection augments production of nitric oxide (NO) in epithelial cells and causes the<jats:italic>S<\/jats:italic>-nitrosylation of dynamin2, mainly on cysteine 86 (C86) and 607 (C607) residues. Forced overexpression of dynamin2 bearing C86A and\/or C607A mutations decreases Ad5 infection. Diminishing NO synthesis by RNAi-induced knockdown of endogenous endothelial NO synthase (eNOS) expression attenuates virus infection of target cells. Ad5 infection promotes the kinetically dynamic<jats:italic>S<\/jats:italic>-nitrosylation of dynamin2 and eNOS: there is a rapid decrease in eNOS<jats:italic>S<\/jats:italic>-nitrosylation and a concomitant increase in the dynamin2<jats:italic>S<\/jats:italic>-nitrosylation. These results support the hypothesis that dynamin2<jats:italic>S<\/jats:italic>-nitrosylation following eNOS activation facilitates adenovirus infection of host epithelial cells.<\/jats:p>","DOI":"10.1099\/vir.0.042713-0","type":"journal-article","created":{"date-parts":[[2012,7,13]],"date-time":"2012-07-13T16:21:03Z","timestamp":1342196463000},"page":"2109-2117","source":"Crossref","is-referenced-by-count":15,"title":["Dynamin2 S-nitrosylation regulates adenovirus type 5 infection of epithelial cells"],"prefix":"10.1099","volume":"93","author":[{"given":"Zhimin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Urology and Prostate Disease Center, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Urology and Prostate Disease Center, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Frilot","sequence":"additional","affiliation":[{"name":"Department of Pathology, Georgia Health Sciences University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehia","family":"Daaka","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Urology and Prostate Disease Center, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.3.1185"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201499200"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211926200"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.2741\/ben"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5304.1320"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.20.120186.000401"},{"key":"r7","doi-asserted-by":"crossref","first-page":"14249","DOI":"10.1074\/jbc.M006258200","article-title":"Direct interaction between endothelial nitric-oxide synthase and dynamin-2. 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