{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:48:11Z","timestamp":1791150491226,"version":"4.1.0"},"reference-count":15,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"<jats:p>Epstein\u2013Barr virus (EBV), a gammaherpesvirus, infects &gt;90\u200a% of the world\u2019s population. Primary infection by EBV can lead to infectious mononucleosis, and EBV persistence is associated with several malignancies. Despite its importance for human health, little structural information is available on EBV. Here we report the purification of the EBV capsid by CsCl- or sucrose density-gradient centrifugation. Cryo-electron microscopy and image analysis resulted in two slightly different three-dimensional structures at about 20 \u00c5 resolution. These structures were compared with that of human herpesvirus 8, another gammaherpesvirus. CsCl-gradient purification leads to the removal of part of the triplex complex around the fivefold axes, whereas the complexes between hexons remained in place. This may be due to local differences in stability resulting from variation in quasi-equivalent interactions between pentons and hexons compared with those between hexons only.<\/jats:p>","DOI":"10.1099\/vir.0.043265-0","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T09:52:43Z","timestamp":1337248363000},"page":"1769-1773","source":"Crossref","is-referenced-by-count":23,"title":["Three-dimensional structure of the Epstein\u2013Barr virus capsid"],"prefix":"10.1099","volume":"93","author":[{"given":"Raphaele","family":"Germi","sequence":"first","affiliation":[{"name":"CHU de Grenoble, Grenoble, France"},{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Effantin","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Grossi","sequence":"additional","affiliation":[{"name":"CHU de Grenoble, Grenoble, France"},{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob W. H.","family":"Ruigrok","sequence":"additional","affiliation":[{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Morand","sequence":"additional","affiliation":[{"name":"CHU de Grenoble, Grenoble, France"},{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Schoehn","sequence":"additional","affiliation":[{"name":"CNRS \u2013 Institut de Biologie Structurale\u2013Jean-Pierre Ebel, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France"},{"name":"Unit for Virus Host Cell Interactions, UMI 3265, Universit\u00e9 Joseph Fourier-EMBL-CNRS, Grenoble, France"},{"name":"UJF-Grenoble-1 \u2013 Institut de Biologie Structurale\u2013Jean-Pierre Ebel, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France"},{"name":"CEA \u2013 Institut de Biologie Structurale\u2013Jean-Pierre Ebel, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1a","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1998.4055"},{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1998.4055"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.12.023"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090284"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01733-08"},{"key":"r6","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1128\/JVI.1.6.1203-1206.1967","article-title":"Replication of herpes-type virus in a Burkitt lymphoma cell line","volume":"1","author":"Hinuma","year":"1967","journal-title":"J Virol"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0321"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2001.iep190.x"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02393-07"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032015"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.6.2879-2890.2001"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3991(87)90078-7"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01565-10"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.24.13182-13193.2003"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5467.877"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.043265-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T19:32:19Z","timestamp":1603740739000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.043265-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,1]]},"references-count":15,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.043265-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,1]]}}}