{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:25:48Z","timestamp":1773404748351,"version":"3.50.1"},"reference-count":44,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:p>Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E<jats:sup>123S<\/jats:sup>); however, JEV with an asparagine residue (E<jats:sup>123N<\/jats:sup>) has also been isolated. To compare the growth properties and pathogenicity of E<jats:sup>123S<\/jats:sup>and E<jats:sup>123N<\/jats:sup>JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E<jats:sup>S123N<\/jats:sup>) in the E<jats:sup>123S<\/jats:sup>-type strain Mie\/41\/2002 background. The growth rate of rJEV-Mie41-E<jats:sup>S123N<\/jats:sup>was similar to that of Mie\/41\/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie\/41\/2002) and rJEV-Mie41-E<jats:sup>S123N<\/jats:sup>. Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties<jats:italic>in vitro<\/jats:italic>or on pathogenicity<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/vir.0.044925-0","type":"journal-article","created":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T00:44:05Z","timestamp":1350002645000},"page":"90-96","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein"],"prefix":"10.1099","volume":"94","author":[{"given":"Yukie","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan"},{"name":"Department of Pathology, Immunology and Microbiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, 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