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In this study, we performed a pseudotyped virus assay using G-deficient rabies virus and demonstrated that single-<jats:italic>N<\/jats:italic>-glycan additions to the G protein of street rabies virus strain 1088, which are found in adapted strains, enhanced virus production in neural and non-neural cell lines, while additions to Asn<jats:sup>194<\/jats:sup>or Asn<jats:sup>247<\/jats:sup>enhanced production greatly. Moreover, we found that<jats:italic>N<\/jats:italic>-glycan additions at Asn<jats:sup>194<\/jats:sup>or Asn<jats:sup>247<\/jats:sup>facilitated the production of cell-associated virus. In contrast, deletion of the sequon at Asn<jats:sup>37<\/jats:sup>reduced viral production, while a deletion at Asn<jats:sup>319<\/jats:sup>resulted in extensive loss of production. Furthermore, G proteins lacking an<jats:italic>N<\/jats:italic>-glycan at Asn<jats:sup>319<\/jats:sup>failed to fold into their correct structure and lost their fusion activity, indicating that Asn<jats:sup>319<\/jats:sup><jats:italic>N<\/jats:italic>-glycosylation is important for the functional expression of street virus G proteins.<\/jats:p>","DOI":"10.1099\/vir.0.047852-0","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T04:36:31Z","timestamp":1351744591000},"page":"270-275","source":"Crossref","is-referenced-by-count":27,"title":["Addition of a single N-glycan to street rabies virus glycoprotein enhances virus production"],"prefix":"10.1099","volume":"94","author":[{"given":"Kentaro","family":"Yamada","sequence":"first","affiliation":[{"name":"Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"},{"name":"Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daichi","family":"Nonaka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muneshin","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiko","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Kawazato","sequence":"additional","affiliation":[{"name":"Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Nishizono","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"},{"name":"Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.17.8096-8104.2001"},{"key":"r2","doi-asserted-by":"crossref","first-page":"3742","DOI":"10.1128\/JVI.71.5.3742-3750.1997","article-title":"Folding of rabies virus glycoprotein: epitope acquisition and interaction with endoplasmic reticulum chaperones","volume":"71","author":"Gaudin","year":"1997","journal-title":"J Virol"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90449-Y"},{"key":"r4","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1348-0421.2002.tb02719.x","article-title":"Studies on the escape mutants of rabies virus which are resistant to neutralization by a highly conserved conformational epitope-specific monoclonal antibody #1-46-12","volume":"46","author":"Irie","year":"2002","journal-title":"Microbiol Immunol"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.19.9121-9128.2001"},{"key":"r6","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1348-0421.2003.tb03424.x","article-title":"Improved recovery of rabies virus from cloned cDNA using a vaccinia virus-free reverse genetics system","volume":"47","author":"Ito","year":"2003","journal-title":"Microbiol Immunol"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.07.066"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvm021"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14756"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201000091"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(97)00080-4"},{"key":"r12","doi-asserted-by":"crossref","first-page":"10690","DOI":"10.1016\/S0021-9258(19)50073-4","article-title":"N-linked glycosylation of rabies virus glycoprotein. 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