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The lack of efficient cell culture models limits studies on molecular and cellular aspects of HEV infection and species barrier crossing. The present study reports on the development of two new <jats:italic>in vitro<\/jats:italic> models of HEV replication using a human hepatoma-derived cell line, HepaRG, and a porcine embryonic stem cell-derived cell line, PICM-19. These two cell lines have morphological and functional properties similar to primary hepatocytes. These <jats:italic>in vitro<\/jats:italic> culture systems support HEV replication and release of encapsidated RNA. These new models represent a powerful tool for studying the viral replication cycle, species barrier crossing and virulence factors.<\/jats:p>","DOI":"10.1099\/vir.0.049858-0","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T01:45:15Z","timestamp":1353635115000},"page":"549-558","source":"Crossref","is-referenced-by-count":58,"title":["New models of hepatitis E virus replication in human and porcine hepatocyte cell lines"],"prefix":"10.1099","volume":"94","author":[{"given":"Sophie","family":"Rog\u00e9e","sequence":"first","affiliation":[{"name":"UMR 1161 Virology, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, 94706 Maisons-Alfort, France"},{"name":"UMR 1161 Virology, INRA, 94706 Maisons-Alfort, France"},{"name":"UMR 1161 Virology, ANSES, Laboratoire de Sant\u00e9 Animale, 94706 Maisons-Alfort, 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