{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:11:03Z","timestamp":1789985463322,"version":"4.0.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:p>We describe the isolation and characterization of a novel flavivirus, isolated from a pool of <jats:italic>Culex<\/jats:italic> (<jats:italic>Melanoconion<\/jats:italic>) <jats:italic>ocossa<\/jats:italic> Dyar and Knab mosquitoes collected in 2009 in an urban area of the Amazon basin city of Iquitos, Peru. Flavivirus infection was detected by indirect immunofluorescent assay of inoculated C6\/36 cells using polyclonal flavivirus antibodies (St. Louis encephalitis virus, yellow fever virus and dengue virus type 1) and confirmed by RT-PCR. Based on partial sequencing of the E and NS5 gene regions, the virus isolate was most closely related to the mosquito-borne flaviviruses but divergent from known species, with less than 45 and 71\u200a% pairwise amino acid identity in the E and NS5 gene products, respectively. Phylogenetic analysis of E and NS5 amino acid sequences demonstrated that this flavivirus grouped with mosquito-borne flaviviruses, forming a clade with Nounan\u00e9 virus (NOUV). Like NOUV, no replication was detected in a variety of mammalian cells (Vero-76, Vero-E6, BHK, LLCMK, MDCK, A549 and RD) or in intracerebrally inoculated newborn mice. We tentatively designate this genetically distinct flavivirus as representing a novel species, Nanay virus, after the river near where it was first detected.<\/jats:p>","DOI":"10.1099\/vir.0.050575-0","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T05:12:58Z","timestamp":1363842778000},"page":"1266-1272","source":"Crossref","is-referenced-by-count":37,"title":["Characterization of a novel flavivirus isolated from Culex (Melanoconion) ocossa mosquitoes from Iquitos, Peru"],"prefix":"10.1099","volume":"94","author":[{"given":"Julio","family":"Evangelista","sequence":"first","affiliation":[{"name":"Virology Department, U.S. Naval Medical Research Unit No. 6, Lima and Iquitos, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristhopher","family":"Cruz","sequence":"additional","affiliation":[{"name":"Virology Department, U.S. Naval Medical Research Unit No. 6, Lima and Iquitos, 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