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In this study, we describe the rescue of recombinant arenaviruses from human 293T cells and Vero cells, an FDA-approved line for vaccine development. We also describe the generation of novel vectors that mediate synthesis of both negative-sense genome RNA and positive-sense mRNA species of lymphocytic choriomeningitis virus (LCMV) directed by the human RNA polymerases I and II, respectively, within the same plasmid. This approach reduces by half the number of vectors required for arenavirus rescue, which could facilitate virus rescue in cell lines approved for human vaccine production but that cannot be transfected at high efficiencies. We have shown the feasibility of this approach by rescuing both the Old World prototypic arenavirus LCMV and the live-attenuated vaccine Candid#1 strain of the New World arenavirus Jun\u00edn. Moreover, we show the feasibility of using these novel strategies for efficient rescue of recombinant tri-segmented both LCMV and Candid#1.<\/jats:p>","DOI":"10.1099\/vir.0.051102-0","type":"journal-article","created":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T03:45:22Z","timestamp":1359603922000},"page":"1175-1188","source":"Crossref","is-referenced-by-count":48,"title":["Arenavirus reverse genetics for vaccine development"],"prefix":"10.1099","volume":"94","author":[{"given":"Emilio","family":"Ortiz-Ria\u00f1o","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benson Yee Hin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Carlos de la Torre","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbial Science, Scripps Research Institute, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Mart\u00ednez-Sobrido","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00276-09"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02556-10"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01837-10"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.1.197"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(91)90018-U"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01144-12"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.05.033"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00151-11"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.2.664"},{"key":"r10","first-page":"1792","article-title":"Arenaviridae: the viruses and their replication","volume-title":"Fields Virology","volume":"vol. 2","author":"Buchmeier","year":"2007"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.015172-0"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.7.3293-3300.2000"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.09.008"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00429-11"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-127"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82452-0"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000047"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8633"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900088106"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.01.013"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02102-10"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-009-0422-9"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa053240"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600652103"},{"key":"r25","doi-asserted-by":"crossref","first-page":"9679","DOI":"10.1128\/JVI.73.11.9679-9682.1999","article-title":"Rescue of influenza A virus from recombinant DNA","volume":"73","author":"Fodor","year":"1999","journal-title":"J Virol"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.47.100193.004001"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9508"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11538-11547.2000"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701849104"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01213-08"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.2008\/004606-0"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.24.13793-13803.2004"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1016\/0959-437X(95)80035-2"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213362"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.0140"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199010183231607"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1086\/322620"},{"key":"r38","first-page":"486","article-title":"Lymphocytic choriomeningitis virus. 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