{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:05:31Z","timestamp":1761581131383},"reference-count":35,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:p>Picobirnavirus (PBV) belongs to the family <jats:italic>Picobirnaviridae<\/jats:italic>. Picobirnaviruses contain a bisegmented dsRNA genome that is non-enveloped. A total of 85 pooled faecal samples were collected from the poultry of 37 farms from the Metropolitan Mesoregion of Bel\u00e9m (MMB), Par\u00e1 state, Brazil. The viral RNA from each sample was analysed by PAGE and reverse transcriptase PCR (RT-PCR). For each county affected, at least one positive sample was selected, cloned and sequenced. The samples showed a positivity of 15.3\u200a% (13\/85) by PAGE and 49.4\u200a% (42\/85) by RT-PCR. Sequencing of these strains demonstrated a considerable RdRp gene heterogeneity that ranged from 56.1 to 100\u200a% at the nucleotide level compared with prototypes of different species and water sewage, and from 50.3 to 100\u200a% among themselves. Avian picobirnavirus (AvPBV) was detected in MMB broiler farms and showed a heterogeneous relationship with the prototypes used. This report includes what is believed to be the first gene sequencing of AvPBV in Brazilian broiler chickens.<\/jats:p>","DOI":"10.1099\/vir.0.054783-0","type":"journal-article","created":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T16:03:37Z","timestamp":1381421017000},"page":"117-122","source":"Crossref","is-referenced-by-count":32,"title":["Genogroup I avian picobirnavirus detected in Brazilian broiler chickens: a molecular epidemiology study"],"prefix":"10.1099","volume":"95","author":[{"given":"Ren\u00e9","family":"Ribeiro Silva","sequence":"first","affiliation":[{"name":"Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento, Avenida Almirante Barroso, N\u00famero 1234, CEP: 66093032, Lanagro, Bel\u00e9m\/Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delana Andreza Melo","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Virologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade. 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