{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:03:58Z","timestamp":1790507038569,"version":"4.1.0"},"reference-count":22,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:p>Dengue virus (DENV) infection affects millions of people annually and has the potential to cause fatal haemorrhagic fever and shock. Although the underlying pathogenesis of severe dengue illness is still unclear, current evidence suggests that severe disease progression has an immunological basis. In this study, we investigated the role of caspase-1 during host\u2013pathogen interactions within DENV-infected human monocytes. Using DENV-infected primary monocytes, we examined caspase-1 at various levels of gene expression and probed for potential immune consequences mediated by caspase-1 such as secretion of pro-inflammatory IL-1\u03b2 and pyroptotic cell death. We report that DENV-infected monocytes upregulated functional caspase-1 mRNA and pro-caspase-1 activation as a late response to infection. In addition, we found that caspase-1 is responsible for IL-1\u03b2 secretion and pyroptosis of DENV-infected monocytes. Together, our results show that late caspase-1 activation within DENV-infected monocytes can contribute to pro-inflammatory outcomes that might play a role in dengue immunopathogenesis.<\/jats:p>","DOI":"10.1099\/vir.0.055277-0","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T04:52:50Z","timestamp":1374727970000},"page":"2215-2220","source":"Crossref","is-referenced-by-count":69,"title":["Dengue virus-infected human monocytes trigger late activation of caspase-1, which mediates pro-inflammatory IL-1\u03b2 secretion and pyroptosis"],"prefix":"10.1099","volume":"94","author":[{"given":"Ter Yong","family":"Tan","sequence":"first","affiliation":[{"name":"Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, MD4, 5 Science Drive 2, National University of Singapore, Singapore 117597"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin Jang Hann","family":"Chu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, MD4, 5 Science Drive 2, National University of Singapore, Singapore 117597"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740040501"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001410"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02103.x"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.4.811"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001557"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.1907-1916.2005"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1002\/art.24959"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.033"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.49.32895"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1086\/383043"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.16.8408-8419.2002"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21051"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2007.07.002"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007255200"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-2-26"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.10.86"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100008"},{"key":"r19","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.4049\/jimmunol.161.7.3340","article-title":"Generation of biologically active IL-1\u03b2 by matrix metalloproteinases: a novel caspase-1-independent pathway of IL-1\u03b2 processing","volume":"161","author":"Sch\u00f6nbeck","year":"1998","journal-title":"J Immunol"},{"key":"r20","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1182\/blood.V91.2.577","article-title":"Lipopolysaccharide activates caspase-1 (interleukin-1-converting enzyme) in cultured monocytic and endothelial cells","volume":"91","author":"Schumann","year":"1998","journal-title":"Blood"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00324-06"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-05-430090"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.055277-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T12:07:58Z","timestamp":1688386078000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.055277-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,1]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2013,10,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.055277-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,1]]}}}