{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:42:46Z","timestamp":1791852166941,"version":"4.3.4"},"reference-count":29,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:p>A method for rapid production of single-round infectious particles (SRIPs) of flavivirus would be useful for viral mutagenesis studies. Here, we established a DNA-based production system for SRIPs of flavivirus. We constructed a Japanese encephalitis virus (JEV) subgenomic replicon plasmid, which lacked the C-prM-E (capsid\u2013pre-membrane\u2013envelope) coding region, under the control of the cytomegalovirus promoter. When the JEV replicon plasmid was transiently co-transfected with a JEV C-prM-E expression plasmid into 293T cells, SRIPs were produced, indicating successful <jats:italic>trans<\/jats:italic>-complementation with JEV structural proteins. Equivalent production levels were observed when C and prM\u2013E proteins were provided separately. Furthermore, dengue types 1\u20134, West Nile, yellow fever or tick-borne encephalitis virus prM-E proteins could be utilized for production of chimaeric flavivirus SRIPs, although the production was less efficient for dengue and yellow fever viruses. These results indicated that our plasmid-based system is suitable for investigating the life cycles of flaviviruses, diagnostic applications and development of safer vaccine candidates.<\/jats:p>","DOI":"10.1099\/vir.0.058008-0","type":"journal-article","created":{"date-parts":[[2013,10,5]],"date-time":"2013-10-05T04:33:32Z","timestamp":1380947612000},"page":"60-65","source":"Crossref","is-referenced-by-count":47,"title":["Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon"],"prefix":"10.1099","volume":"95","author":[{"given":"Ryosuke","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Virology II, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dokkyo Medical University School of Medicine, 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