{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:55:32Z","timestamp":1773294932224,"version":"3.50.1"},"reference-count":22,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:p>The emerging arbovirus Chandipura virus (CV) has been implicated in epidemics of acute encephalitis in India with high mortality rates. The isolation of temperature-dependent host-range (<jats:italic>td<\/jats:italic>CE) mutants, which are impaired in growth at 39 \u00b0C in chick embryo (CE) cells but not in monkey cells, highlights a dependence on undetermined host factors. We have characterized three <jats:italic>td<\/jats:italic>CE mutants, each containing one or more coding mutations in the RNA polymerase gene and two containing additional mutations in the attachment protein gene. Using reverse genetics, we showed that a single amino acid change in the virus polymerase of each mutant was responsible for the host-range specificity. In CE cells at the non-permissive temperature, the discrete cytoplasmic replication complexes seen in mammalian cells or at the permissive temperature in CE cells were absent with the <jats:italic>td<\/jats:italic>CE mutants, consistent with the <jats:italic>td<\/jats:italic>CE lesions causing disruption of the replication complexes in a host-dependent manner.<\/jats:p>","DOI":"10.1099\/vir.0.059204-0","type":"journal-article","created":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T04:42:35Z","timestamp":1381898555000},"page":"38-43","source":"Crossref","is-referenced-by-count":2,"title":["The host-range tdCE phenotype of Chandipura virus is determined by mutations in the polymerase gene"],"prefix":"10.1099","volume":"95","author":[{"given":"Emily L.","family":"Stock","sequence":"first","affiliation":[{"name":"School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony C.","family":"Marriott","sequence":"additional","affiliation":[{"name":"Public Health England, Porton Down, Salisbury SP4 0JG, UK"},{"name":"School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Easton","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","first-page":"1295","article-title":"Chandipura: a new Arbovirus isolated in India from patients with febrile illness","volume":"55","author":"Bhatt","year":"1967","journal-title":"Indian J Med Res"},{"key":"r2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1128\/JVI.73.1.251-259.1999","article-title":"Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter","volume":"73","author":"Buchholz","year":"1999","journal-title":"J Virol"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082243699"},{"key":"r4","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4269\/ajtmh.2005.73.566","article-title":"An outbreak of Chandipura virus encephalitis in the eastern districts of Gujarat state, India","volume":"73","author":"Chadha","year":"2005","journal-title":"Am J Trop Med Hyg"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030315"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00211-06"},{"key":"r7","doi-asserted-by":"crossref","first-page":"570","DOI":"10.4269\/ajtmh.1994.50.570","article-title":"First isolations of arboviruses from phlebotomine sand flies in West Africa","volume":"50","author":"Fontenille","year":"1994","journal-title":"Am J Trop Med Hyg"},{"key":"r8","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1128\/JVI.33.1.107-114.1980","article-title":"Temperature-sensitive mutants of Chandipura virus. 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