{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:52:26Z","timestamp":1790776346576,"version":"4.1.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:p>In recent years, novel henipavirus-related sequences have been identified in bats in Africa. To evaluate the potential of African bat henipaviruses to spread in non-bat mammalian cells, we compared the biological functions of the surface glycoproteins G and F of the prototype African henipavirus GH-M74a with those of the glycoproteins of Nipah virus (NiV), a well-characterized pathogenic member of the henipavirus genus. Glycoproteins are central determinants for virus tropism, as efficient binding of henipavirus G proteins to cellular ephrin receptors and functional expression of fusion-competent F proteins are indispensable prerequisites for virus entry and cell-to-cell spread. In this study, we analysed the ability of the GH-M74a G and F proteins to cause cell-to-cell fusion in mammalian cell types readily permissive to NiV or Hendra virus infections. Except for limited syncytium formation in a bat cell line derived from <jats:italic>Hypsignathus monstrosus<\/jats:italic>, HypNi\/1.1 cells, we did not observe any fusion. The highly restricted fusion activity was predominantly due to the F protein. Whilst GH-M74a G protein was found to interact with the main henipavirus receptor ephrin-B2 and induced syncytia upon co-expression with heterotypic NiV F protein, GH-M74a F protein did not cause evident fusion in the presence of heterotypic NiV G protein. Pulse\u2013chase and surface biotinylation analyses revealed delayed F cleavage kinetics with a reduced expression of cleaved and fusion-active GH-M74a F protein on the cell surface. Thus, the F protein of GH-M74a showed a functional defect that is most likely caused by impaired trafficking leading to less efficient proteolytic activation and surface expression.<\/jats:p>","DOI":"10.1099\/vir.0.060632-0","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T04:50:11Z","timestamp":1386046211000},"page":"539-548","source":"Crossref","is-referenced-by-count":29,"title":["Characterization of African bat henipavirus GH-M74a glycoproteins"],"prefix":"10.1099","volume":"95","author":[{"given":"Michael","family":"Weis","sequence":"first","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Behner","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Herrler","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Drosten","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Bonn Medical Centre, Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Felix","family":"Drexler","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Bonn Medical Centre, Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Dietzel","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Maisner","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00469-10"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028131"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504887102"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.22.11186-11198.2002"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.22.11186-11198.2002"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)04299-3"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504598200"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.02.019"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06628-11"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006367"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1796"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.3648"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.10-0567"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025256"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072942"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-010-0366-2"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02458-13"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02696-12"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1086\/647951"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.3201\/eid1508.081237"},{"key":"r21","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1160\/TH09-05-0310","article-title":"Organ- and endotheliotropism of Nipah virus infections in vivo and in vitro","volume":"102","author":"Maisner","year":"2009","journal-title":"Thromb Haemost"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.3201\/eid1611.100501"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002836"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9705-9712.2004"},{"key":"r25","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/nature03838","article-title":"EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus","volume":"436","author":"Negrete","year":"2005","journal-title":"Nature"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020007"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90434-D"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.01.007"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1021\/bi101597k"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05826-11"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07401.x"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-5-163"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.21.9972-9979.2000"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.060632-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T01:17:42Z","timestamp":1603761462000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.060632-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,1]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.060632-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,1]]}}}