{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:58:04Z","timestamp":1791655084147,"version":"4.3.4"},"reference-count":44,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"<jats:p>Hantaviruses are zoonotic viruses that cause life-threatening diseases when transmitted to humans. Severe hantavirus infection is manifested by impairment of renal function, pulmonary oedema and capillary leakage. Both innate and adaptive immune responses contribute to the pathogenesis, but the underlying mechanisms are not fully understood. Here, we showed that galectin-3-binding protein (Gal-3BP) was upregulated as a result of hantavirus infection both<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>. Gal-3BP is a secreted glycoprotein found in human serum, and increased Gal-3BP levels have been reported in chronic viral infections and in several types of cancer. Our<jats:italic>in vitro<\/jats:italic>experiments showed that, whilst Vero E6 cells (an African green monkey kidney cell line) constitutively expressed and secreted Gal-3BP, this protein was detected in primary human cells only as a result of hantavirus infection. Analysis of Gal-3BP levels in serum samples of cynomolgus macaques infected experimentally with hantavirus indicated that hantavirus infection induced Gal-3BP also<jats:italic>in vivo<\/jats:italic>. Finally, analysis of plasma samples collected from patients hospitalized because of acute hantavirus infection showed higher Gal-3BP levels during the acute than the convalescent phase. Furthermore, the Gal-3BP levels in patients with haemorrhagic fever with renal syndrome correlated with increased complement activation and with clinical variables reflecting the severity of acute hantavirus infection.<\/jats:p>","DOI":"10.1099\/vir.0.066837-0","type":"journal-article","created":{"date-parts":[[2014,7,11]],"date-time":"2014-07-11T05:01:04Z","timestamp":1405054864000},"page":"2356-2364","source":"Crossref","is-referenced-by-count":34,"title":["Acute hantavirus infection induces galectin-3-binding protein"],"prefix":"10.1099","volume":"95","author":[{"given":"Jussi","family":"Hepojoki","sequence":"first","affiliation":[{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Strandin","sequence":"additional","affiliation":[{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo","family":"Hetzel","sequence":"additional","affiliation":[{"name":"Veterinary Pathology, Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarja","family":"Sironen","sequence":"additional","affiliation":[{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Klingstr\u00f6m","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska, University Hospital Huddinge, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussi","family":"Sane","sequence":"additional","affiliation":[{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satu","family":"M\u00e4kel\u00e4","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Tampere, Tampere, Finland"},{"name":"Department of Internal Medicine, Tampere University Hospital, University of Tampere, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jukka","family":"Mustonen","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Tampere, Tampere, Finland"},{"name":"Department of Internal Medicine, Tampere University Hospital, University of Tampere, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Meri","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5ke","family":"Lundkvist","sequence":"additional","affiliation":[{"name":"Swedish Institute for Communicable Disease Control, Solna, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli","family":"Vapalahti","sequence":"additional","affiliation":[{"name":"Department of Virology and Immunology, HUSLAB, Hospital District of Helsinki and Uusimaa, Finland"},{"name":"Veterinary Pathology, Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Finland"},{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilkka","family":"Lankinen","sequence":"additional","affiliation":[{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"Vaheri","sequence":"additional","affiliation":[{"name":"Department of Virology and Immunology, HUSLAB, Hospital District of Helsinki and Uusimaa, Finland"},{"name":"Department of Virology, Peptide and Protein Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(96)80076-6"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100762"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.04.019"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01670.x"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00297-12"},{"key":"r6","doi-asserted-by":"crossref","first-page":"674360","DOI":"10.1155\/2012\/674360","article-title":"Elevated VEGF levels in pulmonary edema fluid and PBMCs from patients with acute hantavirus pulmonary syndrome","volume":"2012","author":"Gavrilovskaya","year":"2012","journal-title":"Adv Virol"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192298899"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00576-10"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1023\/B:GLYC.0000014085.00706.d4"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006634530672"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00481-09"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.021006-0"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00057-10"},{"key":"r14","first-page":"311","article-title":"Matrilysin (MMP-7) degrades VE-cadherin and accelerates accumulation of \u03b2-catenin in the nucleus of human umbilical vein endothelial cells","volume":"15","author":"Ichikawa","year":"2006","journal-title":"Oncol Rep"},{"key":"r15","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1177\/153537020623100103","article-title":"Role of matrix metalloproteinase-7 (matrilysin) in human cancer invasion, apoptosis, growth, and angiogenesis","volume":"231","author":"Ii","year":"2006","journal-title":"Exp Biol Med (Maywood)"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00062-09"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2000.029"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4894-4897.2003"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.1.444-449.2002"},{"key":"r20","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1177\/135965350801300114","article-title":"Passive immunization protects cynomolgus macaques against Puumala hantavirus challenge","volume":"13","author":"Klingstr\u00f6m","year":"2008","journal-title":"Antivir Ther"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(20000615)77:4<540::AID-JCB3>3.0.CO;2-I"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1177\/030006051204000519"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-10-111"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb08674.x"},{"key":"r26","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1160\/TH09-09-0640","article-title":"Hantavirus regulation of endothelial cell functions","volume":"102","author":"Mackow","year":"2009","journal-title":"Thromb Haemost"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1086\/342413"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.29"},{"key":"r29","first-page":"1457","article-title":"Elevated serum levels of a 90,000 daltons tumor-associated antigen in cancer and in infection by human immunodeficiency virus (HIV)","volume":"14","author":"Natoli","year":"1994","journal-title":"Anticancer Res"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-132"},{"key":"r31","first-page":"424","article-title":"Complement activation in nephropathia epidemica caused by Puumala hantavirus","volume":"53","author":"Paakkala","year":"2000","journal-title":"Clin Nephrol"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2008.41.11.784"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-012-0936-6"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2011.573500"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01405-10"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003035"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-008-9114-6"},{"key":"r38","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.3.1988"},{"key":"r39","doi-asserted-by":"crossref","first-page":"18401","DOI":"10.1016\/S0021-9258(17)32322-0","article-title":"The secreted tumor-associated antigen 90K is a potent immune stimulator","volume":"269","author":"Ullrich","year":"1994","journal-title":"J Biol Chem"},{"key":"r40","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.07.030"},{"key":"r41","first-page":"307","article-title":"Hantaviruses","volume-title":"Zoonoses: Biology, Clinical Practice and Public Health","author":"Vaheri","year":"2011"},{"key":"r42","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3066"},{"key":"r43","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-4-829"},{"key":"r44","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1128\/JCM.34.1.119-125.1996","article-title":"Antigenic properties and diagnostic potential of puumala virus nucleocapsid protein expressed in insect cells","volume":"34","author":"Vapalahti","year":"1996","journal-title":"J Clin Microbiol"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.066837-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,15]],"date-time":"2023-07-15T05:27:19Z","timestamp":1689398839000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.066837-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,1]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.066837-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,1]]}}}