{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:54:27Z","timestamp":1761663267988},"reference-count":48,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>We demonstrated previously that immunization with a DNA vaccine expressing the Japanese encephalitis virus (JEV) envelope (E) protein conferred a high level of protection through a poorly neutralizing antibody response. Here, we further investigated the role of the IgG subclass in this antibody-dependent protection using cytokine co-immunization and cytokine-deficient mice. A significant difference in IgG2a\/c but not IgG1 was observed between mice that survived or died following a lethal challenge. Correspondingly, the IgG2a\/c response and protection increased in IL-4-deficient mice but decreased in IFN-\u03b3-deficient mice, highlighting the importance of IgG2a\/c. In addition, the restoration of protection and E-specific IgG2a\/c production in IFN-\u03b3-deficient mice by a T helper (Th) type 1-biased intramuscular immunization suggested that IgG2a\/c but not IFN-\u03b3 was the major component for protection. The failure of protection against a direct intracranial challenge indicated that IgG2a\/c-mediated protection was restricted to outside the central nervous system. Consistent with this conclusion, passive transfer of E-specific antisera conferred protection only pre-exposure to JEV. Therefore, our data provided evidence that the IgG subclass plays an important role in protection against JEV, particular in poorly neutralizing E-specific antibodies, and Th1-biased IgG2a\/c confers better protection than Th2-biased IgG1 against JEV.<\/jats:p>","DOI":"10.1099\/vir.0.067280-0","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T02:23:55Z","timestamp":1402367035000},"page":"1983-1990","source":"Crossref","is-referenced-by-count":13,"title":["A poorly neutralizing IgG2a\/c response elicited by a DNA vaccine protects mice against Japanese encephalitis virus"],"prefix":"10.1099","volume":"95","author":[{"given":"Hsin-Wei","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Immunology, China Medical University, Taichung, Taiwan"},{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences, Academia Sinica, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Mei","family":"Hu","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han-Hsuan","family":"Chung","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szu-Hsien","family":"Wu","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County, 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