{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T10:27:54Z","timestamp":1776680874675,"version":"3.51.2"},"reference-count":52,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"<jats:p>We isolated and characterized a novel virulent bacteriophage, IME-EFm1, specifically infecting multidrug-resistant<jats:italic>Enterococcus faecium<\/jats:italic>. IME-EFm1 is morphologically similar to members of the family<jats:italic>Siphoviridae<\/jats:italic>. It was found capable of lysing a wide range of our<jats:italic>E. faecium<\/jats:italic>collections, including two strains resistant to vancomycin. One-step growth tests revealed the host lysis activity of phage IME-EFm1, with a latent time of 30 min and a large burst size of 116 p.f.u. per cell. These biological characteristics suggested that IME-EFm1 has the potential to be used as a therapeutic agent. The complete genome of IME-EFm1 was 42\u200a597 bp, and was linear, with terminally non-redundant dsDNA and a G+C content of 35.2\u200amol%. The termini of the phage genome were determined with next-generation sequencing and were further confirmed by nuclease digestion analysis. To our knowledge, this is the first report of a complete genome sequence of a bacteriophage infecting<jats:italic>E. faecium<\/jats:italic>. IME-EFm1 exhibited a low similarity to other phages in terms of genome organization and structural protein amino acid sequences. The coding region corresponded to 90.7\u200a% of the genome; 70 putative ORFs were deduced and, of these, 29 could be functionally identified based on their homology to previously characterized proteins. A predicted metallo-\u03b2-lactamase gene was detected in the genome sequence. The identification of an antibiotic resistance gene emphasizes the necessity for complete genome sequencing of a phage to ensure it is free of any undesirable genes before use as a therapeutic agent against bacterial pathogens.<\/jats:p>","DOI":"10.1099\/vir.0.067553-0","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T06:40:22Z","timestamp":1406788822000},"page":"2565-2575","source":"Crossref","is-referenced-by-count":40,"title":["Characterization and complete genome sequence analysis of novel bacteriophage IME-EFm1 infecting Enterococcus faecium"],"prefix":"10.1099","volume":"95","author":[{"given":"Yahui","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"},{"name":"School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR 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