{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:11:14Z","timestamp":1790968274937,"version":"4.1.0"},"reference-count":44,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:p>Functional disruption of dendritic cells (DCs) is an important strategy for viral pathogens to evade host defences. Monocytotropic viruses such as classical swine fever virus (CSFV) could employ such a mechanism, since the virus can suppress immune responses and induce apoptosis without infecting lymphocytes. Here, CSFV was shown to infect and efficiently replicate in monocyte- and in bone marrow-derived DCs. Interestingly, the infected DCs displayed neither modulated MHC nor CD80\/86 expression. Stimulation of DCs with IFN-<jats:italic>\u03b1<\/jats:italic>\/TNF-<jats:italic>\u03b1<\/jats:italic>or polyinosinic\u2013polycytidylic acid (pIC) induced phenotypic maturation with increased MHC and CD80\/86 expression, both with mock-treated and infected DCs. In addition, the T cell stimulatory capacity of CSFV-infected DCs was maintained both in a polyclonal T cell stimulation and in specific antigen-presentation assays, requiring antigen uptake and processing. Interestingly, similar to macrophages, CSFV did not induce IFN-<jats:italic>\u03b1<\/jats:italic>responses in these DCs and even suppressed pIC-induced IFN-<jats:italic>\u03b1<\/jats:italic>induction. Other cytokines including interleukin (IL)-6, IL-10, IL-12 and TNF-<jats:italic>\u03b1<\/jats:italic>were not modulated. Taken together, these results demonstrated that CSFV can replicate in DCs and control IFN type I responses, without interfering with the immune reactivity. These results are interesting considering that DC infection with RNA viruses usually results in DC activation.<\/jats:p>","DOI":"10.1099\/vir.0.19716-0","type":"journal-article","created":{"date-parts":[[2004,5,27]],"date-time":"2004-05-27T20:48:15Z","timestamp":1085690895000},"page":"1633-1641","source":"Crossref","is-referenced-by-count":78,"title":["Interaction of classical swine fever virus with dendritic cells"],"prefix":"10.1099","volume":"85","author":[{"given":"C. P.","family":"Carrasco","sequence":"first","affiliation":[{"name":"Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH-3147 Mittelh\u00e4usern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. C.","family":"Rigden","sequence":"additional","affiliation":[{"name":"Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH-3147 Mittelh\u00e4usern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. 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