{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:11:46Z","timestamp":1790914306198,"version":"4.1.0"},"reference-count":35,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:p>The human leukocyte antigen (HLA)-A*2402 is common in Asians. The authors attempted to identify epitopes for HLA-A*2402-restricted, hepatitis C virus (HCV)-specific CD8<jats:sup>+<\/jats:sup> T cells by an enzyme-linked immunospot (ELISpot) assay using peripheral blood CD8<jats:sup>+<\/jats:sup> T cells from HLA-A*2402-positive hepatitis C patients and synthetic HCV peptides based on HLA-A*2402-binding motifs and the amino acid sequence of type 1b HCV. Ten novel epitopes were identified in five of seven HLA-A*2402-positive patients with acute or short-term chronic HCV infection (&lt;3\u2005years), but in none of four with longer-term chronic infection (&gt;10\u2005years). Only one of the ten epitopes proved to be definitely HLA-A*2402-restricted. Another epitope was identified in one of two HLA-A*2402-negative acute hepatitis C patients. In two of the six patients with positive CD8<jats:sup>+<\/jats:sup> T cell responses, the targeted epitopes were multiple. The same epitope was targeted in two patients. When patients with unresolved acute HCV infection were treated with alpha interferon, peripheral blood HCV-specific CD8<jats:sup>+<\/jats:sup> T cells decreased with resolution of the hepatitis. In conclusion, CD8<jats:sup>+<\/jats:sup> T cell responses to HCV infection are heterogeneous. One definite HLA-A*2402-restricted and ten probably non-HLA-A*2402-restricted epitopes were identified. Patients with short-term HCV infection are suitable for searching for novel HCV epitopes, but peripheral blood HCV-specific CD8<jats:sup>+<\/jats:sup> T cells decrease markedly after loss of antigenic stimulation.<\/jats:p>","DOI":"10.1099\/vir.0.79801-0","type":"journal-article","created":{"date-parts":[[2004,5,27]],"date-time":"2004-05-27T20:48:15Z","timestamp":1085690895000},"page":"1521-1531","source":"Crossref","is-referenced-by-count":12,"title":["Identification of novel hepatitis C virus-specific cytotoxic T lymphocyte epitopes by ELISpot assay using peptides with human leukocyte antigen-A*2402-binding motifs"],"prefix":"10.1099","volume":"85","author":[{"given":"Taku","family":"Hakamada","sequence":"first","affiliation":[{"name":"Division of Gastroenterology, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, 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