{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:52Z","timestamp":1787010592009,"version":"build-2736575974"},"reference-count":23,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:p>\n                    Genome segment 9 (Seg-9) of Colorado tick fever virus (CTFV) is 1884\u2005bp long and contains a large open reading frame (ORF; 1845\u2005nt in length overall), although a single in-frame stop codon (at nt 1052\u20131054) reduces the ORF coding capacity by approximately 40\u200a%. However, analyses of highly conserved RNA sequences in the vicinity of the stop codon indicate that it belongs to a class of \u2018leaky terminators\u2019. The third nucleotide positions in codons situated both before and after the stop codon, shows the highest variability, suggesting that both regions are translated during virus replication. This also suggests that the stop signal is functionally leaky, allowing read-through translation to occur. Indeed, both the truncated \u2018termination\u2019 protein and the full-length \u2018read-through\u2019 protein (VP9 and VP9\u2032, respectively) were detected in CTFV-infected cells, in cells transfected with a plasmid expressing only Seg-9 protein products, and in the\n                    <jats:italic>in vitro<\/jats:italic>\n                    translation products from undenatured Seg-9 ssRNA. The ratios of full-length and truncated proteins generated suggest that read-through may be down-regulated by other viral proteins. Western blot analysis of infected cells and purified CTFV showed that VP9 is a structural component of the virion, while VP9\u2032 is a non-structural protein.\n                  <\/jats:p>","DOI":"10.1099\/vir.0.80019-0","type":"journal-article","created":{"date-parts":[[2004,7,21]],"date-time":"2004-07-21T17:20:07Z","timestamp":1090430407000},"page":"2237-2244","source":"Crossref","is-referenced-by-count":20,"title":["Termination and read-through proteins encoded by genome segment 9 of Colorado tick fever virus"],"prefix":"10.1099","volume":"85","author":[{"given":"Fauziah Mohd","family":"Jaafar","sequence":"first","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (EA 3292, IFR 48): EFS Alpes-M\u00e9diterran\u00e9e and Universit\u00e9 de la M\u00e9diterran\u00e9e, Facult\u00e9 de M\u00e9decine de Marseille, Marseille 13005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houssam","family":"Attoui","sequence":"additional","affiliation":[{"name":"Unit\u00e9 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