{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:23:49Z","timestamp":1758925429013},"reference-count":53,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,11,1]]},"abstract":"<jats:p>Human prion diseases, such as Creutzfeldt\u2013Jakob disease (CJD), a lethal, neurodegenerative condition, occur in sporadic, genetic and transmitted forms. CJD is associated with the conversion of normal cellular prion protein (PrP<jats:sup>C<\/jats:sup>) into a protease-resistant isoform (PrP<jats:sup>res<\/jats:sup>). The mechanism of the conversion has not been studied in human cell cultures, due to the lack of a model system. In this study, such a system has been developed by culturing cell lines. Human glioblastoma cell line T98G had no coding-region mutations of the prion protein gene, which was of the 129\u2005M\/V genotype, and expressed endogenous PrP<jats:sup>C<\/jats:sup> constitutively. T98G cells produced a form of proteinase K (PK)-resistant prion protein fragment following long-term culture and high passage number; its deglycosylated form was approximately 18\u2005kDa. The PK-treated PrP<jats:sup>res<\/jats:sup> was detected by immunoblotting with the mAb 6H4, which recognizes residues 144\u2013152, and a polyclonal anti-C-terminal antibody, but not by the mAb 3F4, which recognizes residues 109\u2013112, or the anti-N-terminal mAb HUC2-13. These results suggest that PrP<jats:sup>C<\/jats:sup> was converted into a proteinase-resistant form of PrP<jats:sup>res<\/jats:sup> in T98G cells.<\/jats:p>","DOI":"10.1099\/vir.0.80043-0","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T20:18:20Z","timestamp":1097698700000},"page":"3449-3457","source":"Crossref","is-referenced-by-count":6,"title":["Propagation of a protease-resistant form of prion protein in long-term cultured human glioblastoma cell line T98G"],"prefix":"10.1099","volume":"85","author":[{"given":"Yutaka","family":"Kikuchi","sequence":"first","affiliation":[{"name":"Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoshi","family":"Kakeya","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, 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