{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:37:11Z","timestamp":1789447031095,"version":"build-2803163510"},"reference-count":31,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:p>A CD8<jats:sup>+<\/jats:sup>T-cell memory response to respiratory syncytial virus (RSV) was generated by using a DNA vaccine construct encoding the dominant K<jats:sup>d<\/jats:sup>-restricted epitope from the viral transcription anti-terminator protein M2 (M2<jats:sub>82\u201390<\/jats:sub>), linked covalently to human<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>-microglobulin (<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m). Cutaneous gene-gun immunization of BALB\/c mice with this construct induced an antigen-specific CD8<jats:sup>+<\/jats:sup>T-cell memory. After intranasal RSV challenge, accelerated CD8<jats:sup>+<\/jats:sup>T-cell responses were observed in pulmonary lymph nodes and virus clearance from the lungs was enhanced. The construct induced weaker CD8<jats:sup>+<\/jats:sup>T-cell responses than those elicited with recombinant vaccinia virus expressing the complete RSV M2 protein, but stronger than those induced by a similar DNA construct without the<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m gene. DNA vaccination led to enhanced pulmonary disease after RSV challenge, with increased weight loss and cell recruitment to the lung. Depletion of CD8<jats:sup>+<\/jats:sup>T cells reduced, but did not abolish, enhancement of disease. Mice vaccinated with a construct encoding a class I-restricted lymphocytic choriomeningitis virus epitope and<jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>m suffered more severe weight loss after RSV infection than unvaccinated RSV-infected mice, although RSV-specific CD8<jats:sup>+<\/jats:sup>T-cell responses were not induced. Thus, in addition to specific CD8<jats:sup>+<\/jats:sup>T cell-mediated immunopathology, gene-gun DNA vaccination causes non-specific enhancement of RSV disease without affecting virus clearance.<\/jats:p>","DOI":"10.1099\/vir.0.80098-0","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T21:12:43Z","timestamp":1096060363000},"page":"3017-3026","source":"Crossref","is-referenced-by-count":15,"title":["Gene-gun DNA vaccination aggravates respiratory syncytial virus-induced pneumonitis"],"prefix":"10.1099","volume":"85","author":[{"given":"Christina","family":"Bartholdy","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark"},{"name":"Department of Respiratory Medicine, Imperial College, St Mary's Campus, Norfolk Place, Paddington, London W2 1PG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieslawa","family":"Olszewska","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Imperial College, St Mary's Campus, Norfolk Place, Paddington, London W2 1PG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Stryhn","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan Randrup","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J. 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