{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T07:46:27Z","timestamp":1723362387228},"reference-count":38,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,7,1]]},"abstract":"<jats:p>BALB\/c mice infected with the Moscow strain of<jats:italic>Ectromelia virus<\/jats:italic>(ECTV-MOS) show a large number of apoptotic cells, and an influx of lymphoid cells in the epithelium and substantia propria of conjunctivae, respectively. The presence of ECTV-MOS antigens in the epithelium of conjunctivae significantly upregulates Fas in the epithelial layer and FasL in the suprabasal layer of conjunctiva. Inhibition of FasL with blocking antibodies in cultures of conjunctival cells isolated from ECTV-MOS-infected BALB\/c mice showed that the Fas\/FasL pathway is important in apoptosis of ECTV-MOS-infected cells. The results also showed that the presence of cytokines, in particular interferon (IFN)-<jats:italic>\u03b3<\/jats:italic>, upregulated expression of Fas. Interleukin (IL) 2, 4, 10 and IFN-<jats:italic>\u03b3<\/jats:italic>were produced at the peak of conjunctivitis (at day 15 of infection) with a predominance of IFN-<jats:italic>\u03b3<\/jats:italic>and a small, but significant, production of IL4 and IL10 compared with non-infected animals. These results suggest that not only is Fas\/FasL expression in conjunctiva involved in elimination of migrating Fas<jats:sup>+<\/jats:sup>cells but also plays an important role in the turnover of conjunctival epithelium and thus may be crucial for ECTV spreading to the surrounding environment.<\/jats:p>","DOI":"10.1099\/vir.0.80709-0","type":"journal-article","created":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T18:02:57Z","timestamp":1118858577000},"page":"2007-2018","source":"Crossref","is-referenced-by-count":7,"title":["Mousepox conjunctivitis: the role of Fas\/FasL-mediated apoptosis of epithelial cells in virus dissemination"],"prefix":"10.1099","volume":"86","author":[{"given":"Malgorzata","family":"Krzyzowska","sequence":"first","affiliation":[{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Polanczyk","sequence":"additional","affiliation":[{"name":"Department of Neuroimmunology, School of Medicine, Oregon Health Sciences University, Portland, OR 97201, USA"},{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Bas","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Cymerys","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ada","family":"Schollenberger","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Chiodi","sequence":"additional","affiliation":[{"name":"Microbiology and Tumor Biology Center, Karolinska Institute, Nobels v\u00e4g 16, S-17177 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Niemialtowski","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Division of Virology, Mycology and Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1126\/science.281.5381.1305","article-title":"Death receptors: signaling and modulation","volume":"281","author":"Ashkenazi","year":"1998","journal-title":"Science"},{"key":"R2","first-page":"120","article-title":"Expression of CD40 and CD40 ligand in the human conjunctival epithelium","volume":"41","author":"Bourcier","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1099\/0022-1317-81-4-1087","article-title":"Ectromelia virus virulence factor p28 acts upstream of caspase-3 in response to UV light-induced apoptosis","volume":"81","author":"Brick","year":"2000","journal-title":"J Gen Virol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1006\/exer.1998.0566","article-title":"Expression of Fas-Fas ligand antigens and apoptotic marker APO2.7 by the human conjunctival epithelium. 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