{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:28:47Z","timestamp":1791080927326,"version":"4.1.0"},"reference-count":28,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:p>In order to investigate hepatitis C virus (HCV) persistence and replication in peripheral blood mononuclear cells (PBMC) from a group of haemophilic individuals, HCV production and release to PBMC culture supernatants (SNs) from HCV singly infected patients and HIV\/HCV co-infected patients was studied. HCV RNA<jats:sup>+<\/jats:sup> SNs were found more frequently from HIV\/HCV co-infected individuals (89\u00b75\u200a%) with poor reconstitution of their immune status than from singly HCV-infected patients (57\u200a%) or from HIV\/HCV co-infected individuals with a good response to highly active anti-retroviral therapy (50\u200a%). The presence of the HCV genome in culture SNs was associated with lower CD4<jats:sup>+<\/jats:sup> T-cell counts and with a more severe clinical picture of HIV infection. In spite of prolonged negative HCV viraemia, PBMC from HIV\/HCV co-infected patients released the HCV genome after culture. HCV permissive PBMC allowed generation of HCV productive B cell lines with continuous HCV replication. These findings add further weight to the involvement of PBMCs in persistence of HCV infection and emphasize the role of B lymphocytes as HCV reservoirs.<\/jats:p>","DOI":"10.1099\/vir.0.80882-0","type":"journal-article","created":{"date-parts":[[2005,5,24]],"date-time":"2005-05-24T22:18:24Z","timestamp":1116973104000},"page":"1717-1727","source":"Crossref","is-referenced-by-count":42,"title":["Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients"],"prefix":"10.1099","volume":"86","author":[{"given":"Patricia","family":"Bar\u00e9","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Hematol\u00f3gicas \u201cMariano R Castex\u201d, Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires 1425, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Massud","sequence":"additional","affiliation":[{"name":"Instituto de 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