{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:40:52Z","timestamp":1790800852151,"version":"4.1.0"},"reference-count":69,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,3,1]]},"abstract":"<jats:p>Dystroglycan (DG) is an extracellular matrix receptor necessary for the development of metazoans from flies to humans and is also an entry route for various pathogens.<jats:italic>Lymphocytic choriomeningitis virus<\/jats:italic>(LCMV), a member of the family<jats:italic>Arenaviridae<\/jats:italic>, infects by binding to<jats:italic>\u03b1<\/jats:italic>-DG. Here, the role of cholesterol lipid rafts in infection by LCMV via<jats:italic>\u03b1<\/jats:italic>-DG was investigated. The cholesterol-sequestering drugs methyl-<jats:italic>\u03b2<\/jats:italic>-cyclodextrin (M<jats:italic>\u03b2<\/jats:italic>CD), filipin and nystatin inhibited the infectivity of LCMV selectively, but did not affect infection by vesicular stomatitis virus. Cholesterol loading after depletion with M<jats:italic>\u03b2<\/jats:italic>CD restored infectivity to control levels. DG was not found in lipid rafts identified with the raft marker ganglioside GM1. Treatment with M<jats:italic>\u03b2<\/jats:italic>CD, however, enhanced the solubility of DG. This may reflect the association of DG with cholesterol outside lipid rafts and suggests that association of DG with non-raft cholesterol is critical for infection by LCMV through<jats:italic>\u03b1<\/jats:italic>-DG.<\/jats:p>","DOI":"10.1099\/vir.0.81444-0","type":"journal-article","created":{"date-parts":[[2006,2,13]],"date-time":"2006-02-13T21:58:34Z","timestamp":1139867914000},"page":"673-678","source":"Crossref","is-referenced-by-count":34,"title":["Role of non-raft cholesterol in lymphocytic choriomeningitis virus infection via \u03b1-dystroglycan"],"prefix":"10.1099","volume":"87","author":[{"given":"Waris A.","family":"Shah","sequence":"first","affiliation":[{"name":"Center for Research in Neuroscience and Department of Neurology and Neurosurgery, McGill University, Montr\u00e9al General Hospital Research Institute, 1650 Cedar Avenue, Montr\u00e9al, Qu\u00e9bec H3G 1A4, 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