{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T13:02:00Z","timestamp":1774789320549,"version":"3.50.1"},"reference-count":24,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Tick-borne encephalitis virus<\/jats:italic> (TBEV), a member of the genus <jats:italic>Flavivirus<\/jats:italic>, has a positive-strand RNA genome containing a single open reading frame flanked by non-coding regions (NCRs). <jats:italic>Ixodes ricinus<\/jats:italic> ticks (<jats:italic>n<\/jats:italic>=307) were collected from vegetation in a natural TBEV focus in Belp, Switzerland. The presence and identity of the virus were determined by nested RT-PCR followed by sequencing of the 5\u2032-terminal region that comprises the 5\u2032 NCR and the capsid-encoding region (C). The presence of the western European TBEV subtype (W-TBEV) genome was detected in 14.3\u200a% of the ticks. Nucleotide sequence analysis revealed a high variability of 55.5\u200a%. In particular, four DNA fragments (CS \u2018A\u2019, CS \u2018B\u2019, the folding-stem structure and the start codon) showed substantial heterogeneity, which has the potential of compromising replication, translation and packaging of the viral genome. This variability may reflect a viral strategy to select the fittest RNA molecule to produce a viral infection in the different vertebrate hosts that may be encountered by the ticks. It may also indicate a possible ancient introduction of TBEV to the Belp site. In addition, it may contribute to explaining the annual low incidence of tick-borne encephalitis in the natural focus of Belp, despite the high prevalence of TBEV genomes in ticks.<\/jats:p>","DOI":"10.1099\/vir.0.81783-0","type":"journal-article","created":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T21:51:15Z","timestamp":1153173075000},"page":"2235-2241","source":"Crossref","is-referenced-by-count":22,"title":["Diversity of the population of Tick-borne encephalitis virus infecting Ixodes ricinus ticks in an endemic area of central Switzerland (Canton Bern)"],"prefix":"10.1099","volume":"87","author":[{"given":"Simona","family":"Casati","sequence":"first","affiliation":[{"name":"Istituto Cantonale di Microbiologia, Via Mirasole 22A, 6500 Bellinzona, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lise","family":"Gern","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie, Universit\u00e9 de Neuch\u00e2tel, Rue Emile Argand, 2000 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Europe","volume":"10","author":"Charrel","year":"2004","journal-title":"Clin Microbiol Infect"},{"key":"R4","article-title":"Etude s\u00e9ro\u00e9pid\u00e9miologique de l'enc\u00e9phalite \u00e0 tiques et de la Borreliose de Lyme en Suisse","author":"de Marval","year":"1994"},{"key":"R5","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1086\/515195","article-title":"Tick-borne encephalitis","volume":"28","author":"Dumpis","year":"1999","journal-title":"Clin Infect Dis"},{"key":"R6","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev.ge.22.120188.002513","article-title":"Phylogenies from molecular sequences: inference and reliability","volume":"22","author":"Felsenstein","year":"1988","journal-title":"Annu Rev Genet"},{"key":"R7","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0168-1702(97)01451-2","article-title":"Complete sequence of two tick-borne flaviviruses isolated from Siberia and the UK: analysis and significance of the 5\u2032 and 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selection","volume":"86","author":"Jerzak","year":"2005","journal-title":"J Gen Virol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"6719","DOI":"10.1128\/JVI.75.14.6719-6728.2001","article-title":"Essential role of cyclization sequences in flavivirus RNA replication","volume":"75","author":"Khromykh","year":"2001","journal-title":"J Virol"},{"key":"R13","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1046\/j.1365-2915.1999.00166.x","article-title":"Mixed infection by tick-borne encephalitis virus and Borrelia in ticks","volume":"13","author":"Korenberg","year":"1999","journal-title":"Med Vet Entomol"},{"key":"R14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S1438-4221(02)80006-6","article-title":"TBE foci in Switzerland","volume":"291 (Suppl. 33)","author":"Krech","year":"2002","journal-title":"Int J Med Microbiol"},{"key":"R15","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1093\/bioinformatics\/17.12.1244","article-title":"mega2: molecular evolutionary genetic analysis software","volume":"17","author":"Kumar","year":"2001","journal-title":"Bioinformatics"},{"key":"R16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JVI.72.1.73-83.1998","article-title":"Phylogeny of the genus Flavivirus","volume":"72","author":"Kuno","year":"1998","journal-title":"J Virol"},{"key":"R17","doi-asserted-by":"crossref","first-page":"5240","DOI":"10.1128\/JCM.41.11.5240-5244.2003","article-title":"Comparison of hepatitis C virus genotyping by 5\u2032 noncoding region- and core-based reverse transcriptase PCR assay with sequencing and use of the assay for determining subtype distribution in India","volume":"41","author":"Lole","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/viro.1993.1247","article-title":"Complete genomic sequence of Powassan virus: evaluation of genetic elements in tick-borne versus mosquito-borne 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