{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:22:55Z","timestamp":1762100575880},"reference-count":47,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,11,1]]},"abstract":"<jats:p>A molecular clone of <jats:italic>Japanese encephalitis virus<\/jats:italic> (JE virus) was derived from the JE virus Nakayama strain and used to produce infectious JE virus in cell culture. The engineered JE virus resembled the parental JE virus in cell-culture properties and was related closely to other JE virus strains based on nucleotide sequence analysis. The JE virus clone was used as a genetic background for construction of a chimeric virus containing the structural proteins prM and E of <jats:italic>Dengue virus<\/jats:italic>, serotype 2. The chimeric JE\/dengue 2 virus generated authentic dengue 2 structural proteins as assessed by immunoassays for the dengue E protein. It exhibited a small plaque size and less efficient growth in various cell lines than the parental JE virus. JE\/dengue 2 virus was non-neuroinvasive for young adult mice, but displayed partial neurovirulence at doses up to 4 log p.f.u. given intracerebrally. Immunization of 3-week-old mice with JE\/dengue 2 virus yielded neutralizing-antibody titres against dengue 2 virus and conferred protection against dengue encephalitis caused by neuroadapted dengue 2 virus. A rise in post-challenge neutralizing-antibody titres against dengue 2 virus in surviving mice suggests that immunization is associated with establishment of a memory antibody response in this model. This study demonstrates the capacity of JE virus to serve as a vector for expression of heterologous flavivirus structural proteins. Similar to previous studies with other chimeric flaviviruses, this approach may be useful as a genetic system for engineering experimental vaccines against <jats:italic>Dengue virus<\/jats:italic> and other medically important flaviviruses.<\/jats:p>","DOI":"10.1099\/vir.0.81909-0","type":"journal-article","created":{"date-parts":[[2006,10,9]],"date-time":"2006-10-09T21:51:04Z","timestamp":1160430664000},"page":"3131-3140","source":"Crossref","is-referenced-by-count":14,"title":["Chimeric Japanese encephalitis virus\/dengue 2 virus infectious clone: biological properties, immunogenicity and protection against dengue encephalitis in mice"],"prefix":"10.1099","volume":"87","author":[{"given":"Thomas J.","family":"Chambers","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, St Louis University School of Medicine, 1402 South Grand Avenue, St Louis, MO 63104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoshan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, St Louis University School of Medicine, 1402 South Grand Avenue, St Louis, MO 63104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah A.","family":"Droll","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, St Louis University School of Medicine, 1402 South Grand Avenue, St Louis, MO 63104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, St Louis University School of Medicine, 1402 South Grand Avenue, St Louis, MO 63104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S. 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