{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T16:43:01Z","timestamp":1772469781322,"version":"3.50.1"},"reference-count":56,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,10,1]]},"abstract":"<jats:p>There are marked differences in the clinical expression of diseases associated with porcine circovirus type 2 (PCV2) in the field. The objective of this study was to compare the sequences and pathogenicity of PCV2 isolates from field cases with and without PCV2-associated lesions. Forty-two specific-pathogen-free (SPF) pigs were assigned randomly to three groups of 14 pigs each. At 7\u2005weeks of age, group 1 pigs were mock-inoculated with saline, group 2 pigs were inoculated with PCV2-4838 (isolated from a pig with no evidence of PCV2-associated lymphoid lesions) and group 3 pigs were inoculated with PCV2-40895 (isolated from a pig with PCV2-associated lymphoid lesions and disease). The PCV2-4838 and PCV2-40895 isolates shared approximately 98.9\u200a% nucleotide sequence identity across the entire genome. A total of nine amino acid changes in ORF2 and two amino acid changes in ORF1 were identified between the two isolates. PCV2-4838-inoculated pigs had significantly more genomic copy numbers of PCV2 in their sera at 7\u2005days post-inoculation (p.i.) (<jats:italic>P<\/jats:italic>&lt;0.0001) and significantly fewer genomic copy numbers at 14, 21 and 28\u2005days p.i. (<jats:italic>P<\/jats:italic>&lt;0.05) compared with pigs inoculated with the PCV2-40895 isolate. Microscopic lesions in lymphoid tissues were significantly less severe (<jats:italic>P<\/jats:italic>&lt;0.05) and the amount of PCV2 antigen associated with these lesions was significantly lower (<jats:italic>P<\/jats:italic>&lt;0.05) in pigs inoculated with PCV2-4838. The results of this study suggest that PCV2 isolates from the USA differ in virulence in an SPF pig model.<\/jats:p>","DOI":"10.1099\/vir.0.82099-0","type":"journal-article","created":{"date-parts":[[2006,9,8]],"date-time":"2006-09-08T23:23:46Z","timestamp":1157757826000},"page":"2923-2932","source":"Crossref","is-referenced-by-count":76,"title":["Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence"],"prefix":"10.1099","volume":"87","author":[{"given":"T.","family":"Opriessnig","sequence":"first","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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