{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:43:47Z","timestamp":1778690627962,"version":"3.51.4"},"reference-count":23,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,1]]},"abstract":"<jats:p>The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis virus synthesize the L* protein from an alternative initiation codon. L* is considered to play a key role in viral persistence and demyelination in susceptible strains of mice, although this hypothesis is still controversial. By using a mutant virus that expresses FLAG epitope-tagged L*, it was demonstrated previously that L* is expressed exclusively in neurons <jats:italic>in vivo<\/jats:italic> in the acute phase of infection in the central nervous system (CNS). However, in the mutant virus, the C-H-C-C zinc-binding motif in the leader protein (L) was disrupted by the insertion of the FLAG epitope, resulting in clearance of the virus from the CNS. Therefore, a further two mutant viruses were newly generated, expressing FLAG epitope-tagged L* in which the C-H-C-C zinc-binding motif within L is spared. Both mutant viruses caused persistence and demyelination successfully in spinal cords and enabled us to identify L* immunohistochemically in the demyelinating lesions.<\/jats:p>","DOI":"10.1099\/vir.0.82381-0","type":"journal-article","created":{"date-parts":[[2007,7,10]],"date-time":"2007-07-10T21:03:02Z","timestamp":1184101382000},"page":"2268-2274","source":"Crossref","is-referenced-by-count":6,"title":["Expression of L* protein of Theiler's murine encephalomyelitis virus in the chronic phase of infection"],"prefix":"10.1099","volume":"88","author":[{"given":"Kunihiko","family":"Asakura","sequence":"first","affiliation":[{"name":"Department of Microbiology, Kanazawa Medical University, Ishikawa 920-0293, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harunobu","family":"Murayama","sequence":"additional","affiliation":[{"name":"Division of Pathology, Sendai City Hospital, Miyagi 984-0075, 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