{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:36:48Z","timestamp":1790869008953,"version":"4.1.0"},"reference-count":39,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:p>With the use of increasingly sensitive methods for detection of the abnormal isoform of prion protein (PrP<jats:sup>Sc<\/jats:sup>) and infectivity in prion diseases, it has recently been shown that parts of the peripheral nervous system (PNS) of bovine spongiform encephalopathy (BSE)-affected cattle may become infected. It has been reported that prions spread to the central nervous system (CNS) via the PNS in sheep scrapie, but the pathogenesis of BSE in cattle is less well understood. To determine whether parts of the PNS other than those implicated directly in the hypothetical pathogenetic spread of agent from the intestine to the CNS become involved before or after the CNS is affected, PrP<jats:sup>Sc<\/jats:sup>distribution was investigated by a highly sensitive Western blotting technique in dorsal root ganglia, stellate ganglion, phrenic, radial and sciatic nerves, adrenal gland and CNS of cattle that were inoculated orally with BSE-affected brain and culled sequentially. In experimentally BSE-affected cattle, PrP<jats:sup>Sc<\/jats:sup>was first detected in the CNS and dorsal root ganglia; subsequently, PrP<jats:sup>Sc<\/jats:sup>accumulation was detected in the peripheral nerve trunks. PrP<jats:sup>Sc<\/jats:sup>was also detected in the adrenal glands of cattle that showed clinical signs. No PrP<jats:sup>Sc<\/jats:sup>was detected in the PNS of BSE-negative cattle. This study shows that, with respect to dorsal root ganglia, a paravertebral sympathetic ganglion and the somatic nerves examined, PrP<jats:sup>Sc<\/jats:sup>is detected in the PNS during the disease course at the same time as, or after, it accumulates in the CNS.<\/jats:p>","DOI":"10.1099\/vir.0.82779-0","type":"journal-article","created":{"date-parts":[[2007,5,7]],"date-time":"2007-05-07T17:28:46Z","timestamp":1178558926000},"page":"1850-1858","source":"Crossref","is-referenced-by-count":45,"title":["Prions in the peripheral nerves of bovine spongiform encephalopathy-affected cattle"],"prefix":"10.1099","volume":"88","author":[{"given":"Kentaro","family":"Masujin","sequence":"first","affiliation":[{"name":"Prion Disease Research Center, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Matthews","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency (VLA), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald A. H.","family":"Wells","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency (VLA), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirou","family":"Mohri","sequence":"additional","affiliation":[{"name":"Prion Disease Research Center, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Prion Disease Research Center, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1099\/0022-1317-79-3-601","article-title":"Cerebral targeting indicates vagal spread of infection in hamsters fed with scrapie","volume":"79","author":"Beekes","year":"1998","journal-title":"J Gen Virol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1126\/science.6815801","article-title":"Identification of a protein that purifies with the scrapie prion","volume":"218","author":"Bolton","year":"1982","journal-title":"Science"},{"key":"R3","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1086\/431602","article-title":"Highly bovine spongiform encephalopathy-sensitive transgenic mice confirm the essential restriction of infectivity to the nervous system in clinically diseased cattle","volume":"192","author":"Buschmann","year":"2005","journal-title":"J Infect Dis"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S0140-6736(96)90638-8","article-title":"New variant of Creutzfeldt\u2013Jakob disease in a 26-year-old French man","volume":"347","author":"Chazot","year":"1996","journal-title":"Lancet"},{"key":"R5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/385197a0","article-title":"Predicting the CJD epidemic in humans","volume":"385","author":"Cousens","year":"1997","journal-title":"Nature"},{"key":"R6","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1001\/archneur.61.5.747","article-title":"Pathologic prion protein spreading in the peripheral nervous system of a patient with sporadic Creutzfeldt-Jakob disease","volume":"61","author":"Favereaux","year":"2004","journal-title":"Arch Neurol"},{"key":"R7","first-page":"387","article-title":"Neuropathology of scrapie: precision of the lesions and their diversity. In Slow Transmissible Diseases of the Nervous System","author":"Fraser","year":"1979"},{"key":"R8","first-page":"145","article-title":"Transmission to mice, sheep and goat and bioassay of bovine tissues","volume-title":"In Transmissible Spongiform Encephalopathies","author":"Fraser","year":"1994"},{"key":"R9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1006\/nbdi.1996.0019","article-title":"Detection of scrapie agent in the peripheral nervous system of a diseased sheep","volume":"3","author":"Groschup","year":"1996","journal-title":"Neurobiol Dis"},{"key":"R10","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s004010051108","article-title":"Deposition of disease-associated prion protein involves the peripheral nervous system in experimental scrapie","volume":"98","author":"Groschup","year":"1999","journal-title":"Acta Neuropathol (Berl"},{"key":"R11","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1038\/nm922","article-title":"The sympathetic nervous system is involved in variant Creutzfeldt\u2013Jakob disease","volume":"9","author":"Haik","year":"2003","journal-title":"Nat Med"},{"key":"R12","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1292\/jvms.66.515","article-title":"Effect of tissue deterioration on postmortem BSE diagnosis by immunobiochemical detection of an abnormal isoform of prion protein","volume":"66","author":"Hayashi","year":"2004","journal-title":"J Vet Med Sci"},{"key":"R13","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/j.bbrc.2005.01.065","article-title":"The N-terminal cleavage site of PrPSc from BSE differs from that of PrPSc from scrapie","volume":"328","author":"Hayashi","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"R14","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0002-9440(10)63105-7","article-title":"Peripheral tissue involvement in sporadic, iatrogenic, and variant Creutzfeldt\u2013Jakob disease: an immunohistochemical, quantitative, and biochemical study","volume":"164","author":"Head","year":"2004","journal-title":"Am J Pathol"},{"key":"R15","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1136\/jnnp.2003.035154","article-title":"Involvement of the peripheral nervous system in human prion diseases including dural graft associated Creutzfeldt\u2013Jakob disease","volume":"76","author":"Ishida","year":"2005","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"R16","doi-asserted-by":"crossref","first-page":"p","DOI":"10.1007\/4-431-29402-3_17","article-title":"PrPSc distribution of a natural case of bovine spongiform encephalopathy","volume-title":"In Prions: Food and Drug Safety","author":"Iwamaru","year":"2005"},{"key":"R17","doi-asserted-by":"crossref","first-page":"100","DOI":"10.7883\/yoken.JJID.2006.100","article-title":"Distribution of PrPSc in cattle with bovine spongiform encephalopathy slaughtered at abattoirs in Japan","volume":"59","author":"Iwata","year":"2006","journal-title":"Jpn J Infect Dis"},{"key":"R18","volume-title":"Bovine Spongiform Encephalopathy in Great Britain \u2013 A Progress Report","year":"1995"},{"key":"R19","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1093\/brain\/101.2.333","article-title":"Subacute spongiform encephalopathy (Creutzfeldt\u2013Jakob disease). The nature and progression of spongiform change","volume":"101","author":"Masters","year":"1978","journal-title":"Brain"},{"key":"R20","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0304-3940(99)00223-2","article-title":"Pathological PrP is abundant in sympathetic and sensory ganglia of hamsters fed with scrapie","volume":"265","author":"McBride","year":"1999","journal-title":"Neurosci Lett"},{"key":"R21","doi-asserted-by":"crossref","first-page":"9320","DOI":"10.1128\/JVI.75.19.9320-9327.2001","article-title":"Early spread of scrapie from the gastrointestinal tract to the central nervous system involves autonomic fibers of the splanchnic and vagus nerves","volume":"75","author":"McBride","year":"2001","journal-title":"J Virol"},{"key":"R22","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1126\/science.1675487","article-title":"Molecular biology of prion diseases","volume":"252","author":"Prusiner","year":"1991","journal-title":"Science"},{"key":"R23","doi-asserted-by":"crossref","first-page":"6942","DOI":"10.1021\/bi00269a050","article-title":"Further purification and characterization of scrapie prions","volume":"21","author":"Prusiner","year":"1982","journal-title":"Biochemistry"},{"key":"R24","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1038\/nbt748","article-title":"Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice","volume":"20","author":"Safar","year":"2002","journal-title":"Nat Biotechnol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"14279","DOI":"10.1073\/pnas.94.26.14279","article-title":"Identification of a prion protein epitope modulating transmission of bovine spongiform encephalopathy prions to transgenic mice","volume":"94","author":"Scott","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R26","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1111\/j.1348-0421.2005.tb03660.x","article-title":"Rapid PrPSc detection in lymphoid tissue and application to scrapie surveillance of fallen stock in Japan: variable PrPSc accumulation in palatal tonsil in natural scrapie","volume":"49","author":"Shimada","year":"2005","journal-title":"Microbiol Immunol"},{"key":"R27","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1136\/vr.152.13.387","article-title":"Detection of disease-specific PrP in the distal ileum of cattle exposed orally to the agent of bovine spongiform encephalopathy","volume":"152","author":"Terry","year":"2003","journal-title":"Vet Rec"},{"key":"R28","first-page":"57","article-title":"Pathogenesis of natural scrapie in sheep","volume":"16","author":"van Keulen","year":"2000","journal-title":"Arch Virol Suppl"},{"key":"R29","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-3-0348-7949-1_5","article-title":"Animal models of transmissible bovine spongiform encephalopathies: experimental infection, observation and tissue collection","volume-title":"In Techniques in Prion Research","author":"Wells","year":"2004"},{"key":"R30","first-page":"595","article-title":"Bovine spongiform encephalopathy and related diseases","volume-title":"In Prion Biology and Diseases","author":"Wells","year":"2004"},{"key":"R31","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1136\/vr.135.2.40","article-title":"Infectivity in the ileum of cattle challenged orally with bovine spongiform encephalopathy","volume":"135","author":"Wells","year":"1994","journal-title":"Vet Rec"},{"key":"R32","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/978-1-4612-2406-8_3","article-title":"Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy","volume-title":"In Bovine Spongiform Encephalopathy: The BSE Dilemma","author":"Wells","year":"1996"},{"key":"R33","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1136\/vr.142.5.103","article-title":"Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy (BSE): an update","volume":"142","author":"Wells","year":"1998","journal-title":"Vet Rec"},{"key":"R34","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1136\/vr.144.11.292","article-title":"Limited detection of sternal bone marrow infectivity in the clinical phase of experimental bovine spongiform encephalopathy (BSE)","volume":"144","author":"Wells","year":"1999","journal-title":"Vet Rec"},{"key":"R35","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1136\/vr.156.13.401","article-title":"Pathogenesis of experimental bovine spongiform encephalopathy: preclinical infectivity in tonsil and observations on the distribution of lingual tonsil in slaughtered cattle","volume":"156","author":"Wells","year":"2005","journal-title":"Vet Rec"},{"key":"R36","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1099\/vir.0.82421-0","article-title":"Bovine spongiform encephalopathy: the effect of oral exposure dose on attack rate and incubation period in cattle","volume":"88","author":"Wells","year":"2007","journal-title":"J Gen Virol"},{"key":"R37","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/S0140-6736(96)91412-9","article-title":"A new variant of Creutzfeldt\u2013Jakob disease in the UK","volume":"347","author":"Will","year":"1996","journal-title":"Lancet"},{"key":"R38","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1002\/ana.410430611","article-title":"Descriptive epidemiology of Creutzfeldt\u2013Jakob disease in six European countries, 1993\u20131995. . EU Collaborative Study Group for CJD","volume":"43","author":"Will","year":"1998","journal-title":". Ann Neurol"},{"key":"R39","doi-asserted-by":"crossref","first-page":"11265","DOI":"10.1074\/jbc.M008734200","article-title":"In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies","volume":"276","author":"Yokoyama","year":"2001","journal-title":"J Biol Chem"}],"container-title":["Journal of General Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.82779-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T03:59:14Z","timestamp":1707883154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jgv\/10.1099\/vir.0.82779-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,1]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/vir.0.82779-0","relation":{},"ISSN":["0022-1317","1465-2099"],"issn-type":[{"value":"0022-1317","type":"print"},{"value":"1465-2099","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,1]]}}}