{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:21:51Z","timestamp":1790853711485,"version":"4.1.0"},"reference-count":35,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"abstract":"<jats:p>Epstein\u2013Barr virus (EBV) latent membrane protein 2A (LMP2A) blocks B-cell receptor (BCR) signalling after BCR cross-linking to inhibit activation of lytic EBV, and ectopically expressed LMP2B negatively regulates LMP2A. Here, it is demonstrated that silencing of<jats:italic>LMP2B<\/jats:italic>in EBV-harbouring Burkitt's lymphoma Akata cells results in reduced expression of EBV immediate-early lytic<jats:italic>BZLF1<\/jats:italic>gene mRNA and late lytic gp350\/220 protein upon BCR cross-linking. Similarly, reduction of lytic EBV activation was observed in Akata cells overexpressing LMP2A. In contrast, silencing of<jats:italic>LMP2A<\/jats:italic>expression resulted in higher lytic EBV mRNA and protein expression in BCR cross-linked Akata cells. These observations indicate a role for LMP2B distinct from that of LMP2A in regulation of lytic EBV activation in the host cell and support the hypothesis that LMP2B exhibits a negative-regulatory effect on the ability of LMP2A to maintain EBV latency by preventing the switch to lytic replication.<\/jats:p>","DOI":"10.1099\/vir.0.82790-0","type":"journal-article","created":{"date-parts":[[2007,4,5]],"date-time":"2007-04-05T19:24:40Z","timestamp":1175801080000},"page":"1454-1459","source":"Crossref","is-referenced-by-count":15,"title":["Silencing of latent membrane protein 2B reduces susceptibility to activation of lytic Epstein\u2013Barr virus in Burkitt's lymphoma Akata cells"],"prefix":"10.1099","volume":"88","author":[{"given":"Markus P.","family":"Rechsteiner","sequence":"first","affiliation":[{"name":"Experimental Infectious Diseases and Cancer Research, University Children's Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Berger","sequence":"additional","affiliation":[{"name":"Experimental Infectious Diseases and Cancer Research, University Children's Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Weber","sequence":"additional","affiliation":[{"name":"Experimental Infectious Diseases and Cancer Research, University Children's Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrg A.","family":"Sigrist","sequence":"additional","affiliation":[{"name":"Experimental Infectious Diseases and Cancer Research, University Children's Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Nadal","sequence":"additional","affiliation":[{"name":"Experimental Infectious Diseases and Cancer Research, University Children's 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