{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:56:33Z","timestamp":1761807393901},"reference-count":39,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,10,1]]},"abstract":"<jats:p>The unconventional nature of the infectious agent of prion diseases poses a challenge to conventional infection control methodologies. The extraneural tissue distribution of variant and sporadic Creutzfeldt\u2013Jakob disease has increased concern regarding the risk of prion disease transmission via general surgical procedures and highlighted the need for decontamination procedures that can be incorporated into routine processing. In this study, the ability of preparations of enzymatic medical instrument cleaners to reduce the infectivity associated with a rodent-adapted strain of human prion disease, previously reported to be resistant to decontamination, was tested. Efficient degradation of the disease-associated prion protein by enzymatic cleaning preparations required high treatment temperatures (50\u201360\u2005\u00b0C). Standard decontamination methods (1\u2005M NaOH for 1\u2005h or autoclaving at 134\u2005\u00b0C for 18\u2005min) reduced infectivity associated with the human-derived prion strain by less than 3\u2005log<jats:sub>10<\/jats:sub>\u2005LD<jats:sub>50<\/jats:sub>. In contrast, a 30\u2005min treatment with the optimized enzymatic cleaning preparation protocols reduced infectivity by more than 3\u2005log<jats:sub>10<\/jats:sub>\u2005LD<jats:sub>50<\/jats:sub> and when used in conjunction with autoclave cycles eliminated detectable levels of infectivity. The development of prion decontamination procedures that are compatible with routine cleaning and sterilization of medical and surgical instruments may reduce the risk of the transmission of prion disease in general surgery.<\/jats:p>","DOI":"10.1099\/vir.0.82961-0","type":"journal-article","created":{"date-parts":[[2007,9,13]],"date-time":"2007-09-13T21:48:51Z","timestamp":1189720131000},"page":"2905-2914","source":"Crossref","is-referenced-by-count":34,"title":["Enzymatic detergent treatment protocol that reduces protease-resistant prion protein load and infectivity from surgical-steel monofilaments contaminated with a human-derived prion strain"],"prefix":"10.1099","volume":"88","author":[{"given":"Victoria A.","family":"Lawson","sequence":"first","affiliation":[{"name":"Department of Pathology, The University of Melbourne and Mental Health Research Institute, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James 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