{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:22:24Z","timestamp":1785820944224,"version":"3.56.0"},"reference-count":41,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:p>The attenuated West Nile virus 25A strain (WN25A) was investigated for its neuroinvasive properties in B-cell-deficient (\u03bcMT) mice. After peripheral inoculation, WN25A caused fatal encephalitis in the majority of 6\u20138-week-old mice, characterized by a systemic infection with viraemia, moderate virus burdens in peripheral tissues and a high titre of brain-associated virus. Mice generally succumbed to infection within a few weeks of infection. However, others survived for as long as 10\u2005weeks, and some for even longer. Normal age-matched C57BL\/6 mice showed no signs of illness after inoculation with WN25A virus. Nucleotide sequencing of WN25A viruses recovered from the brains of B-cell-deficient mice revealed that the conserved<jats:italic>N<\/jats:italic>-linked glycosylation site in the viral envelope protein was abolished by substitution of a serine residue at position 155. This was found to be a pseudoreversion relative to the wild-type WN-Israel strain, based on virulence testing of one such brain-associated virus in both B-cell-deficient and normal C57BL\/6 mice. This study provides further characterization of the mouse virulence properties of the attenuated WN25A virus in the context of B-cell deficiency. Replication in these mice does not involve rapid neuroadaptation or reversion of WN25A virus to a neuroinvasive phenotype. Molecular modelling studies suggest a difference in local structure of the E protein associated with either an asparagine or serine residue at position 155 compared with the tyrosine found in the virulent parental WN-Israel virus.<\/jats:p>","DOI":"10.1099\/vir.0.83297-0","type":"journal-article","created":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T18:29:22Z","timestamp":1202927362000},"page":"627-635","source":"Crossref","is-referenced-by-count":13,"title":["West Nile 25A virus infection of B-cell-deficient (\u03bcMT) mice: characterization of neuroinvasiveness and pseudoreversion of the viral envelope protein"],"prefix":"10.1099","volume":"89","author":[{"given":"Thomas J.","family":"Chambers","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1402 South Grand Ave, St Louis, MO 63104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah A.","family":"Droll","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1402 South Grand Ave, St Louis, MO 63104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew H.","family":"Walton","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1402 South Grand Ave, St Louis, MO 63104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1402 South Grand Ave, St Louis, MO 63104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William S. 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