{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:39:21Z","timestamp":1790552361981,"version":"4.1.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:p>Integrated human papillomavirus type 16 (HPV-16) viral loads are currently estimated by quantification with real-time PCR of HPV-16 E6 (RT-E6 and HPV-16 PG) and E2 (RT-E2-1) DNA. We assessed the influence of HPV-16 E2 polymorphism on quantification of integrated HPV-16 DNA in anogenital specimens. HPV-16 E2 was sequenced from 135 isolates (123 from European and 12 from non-European lineages). An assay targeting conserved HPV-16 E2 sequences (RT-E2-2) was optimized and applied with RT-E6 and RT-E2-1 on 139 HPV-16-positive cervicovaginal lavages collected from 74 women [58 human immunodeficiency virus (HIV)-seropositive and 16 HIV-seronegative]. Ratios of HPV-16 copies measured with RT-E2-2 and RT-E2-1 obtained with African 2 (median=3.23, range=1.92\u20133.49) or Asian\u2013American (median=3.78, range=1.47\u201337) isolates were greater than those obtained with European isolates (median=1.02, range=0.64\u20131.80; <jats:italic>P<\/jats:italic>&lt;0.02 for each comparison). The distribution of HPV-16 E2 copies measured in 139 samples with RT-E2-2 (median=6150) and RT-E2-1 (median=8960) were different (<jats:italic>P<\/jats:italic>&lt;0.0001). The risk of high-grade cervical intraepithelial neoplasia (CIN-2,3) compared with women without CIN was increased with higher HPV-16 total [odds ratio (OR)=2.17, 95\u200a% confidence interval (CI)=1.11\u20134.23], episomal (OR=2.14, 95\u200a% CI=1.09\u20134.19), but not for HPV-16 integrated viral load (OR=1.71, 95\u200a% CI=0.90\u20133.26), after controlling for age, race, CD4 count, HIV and HPV-16 polymorphism. The proportion of samples with an E6\/E2 ratio &gt;2 in women without squamous intraepithelial lesion (7 of 35) was similar to that of women with CIN-2,3 (5 of 11, <jats:italic>P<\/jats:italic>=0.24) or CIN-1 (5 of 14, <jats:italic>P<\/jats:italic>=0.50). HPV-16 E2 polymorphism was a significant factor that influenced measures of HPV-16 integrated viral load.<\/jats:p>","DOI":"10.1099\/vir.0.83579-0","type":"journal-article","created":{"date-parts":[[2008,6,17]],"date-time":"2008-06-17T15:47:30Z","timestamp":1213717650000},"page":"1716-1728","source":"Crossref","is-referenced-by-count":15,"title":["Influence of human papillomavirus type 16 (HPV-16) E2 polymorphism on quantification of HPV-16 episomal and integrated DNA in cervicovaginal lavages from women with cervical intraepithelial neoplasia"],"prefix":"10.1099","volume":"89","author":[{"given":"Naoufel","family":"Azizi","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie Mol\u00e9culaire, Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Brazete","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Mol\u00e9culaire, Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Hankins","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Occupational Health Medicine, McGill University, Montreal, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Money","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Mol\u00e9culaire, Centre de Recherche du Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Koushik","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Occupational Health Medicine, McGill University, Montreal, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Rachlis","sequence":"additional","affiliation":[{"name":"Department of Medicine, Sunny Brook Health Science Centre, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Pourreaux","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Occupational Health Medicine, McGill University, Montreal, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Ferenczy","sequence":"additional","affiliation":[{"name":"Department of Pathology and Obstetrics & Gynecology, The Sir Mortimer B. 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